Ene‐reductase: a multifaceted biocatalyst in organic synthesis

T Kumar Roy, R Sreedharan, P Ghosh… - … A European Journal, 2022 - Wiley Online Library
Biocatalysis integrate microbiologists, enzymologists, and organic chemists to access the
repertoire of pharmaceutical and agrochemicals with high chemoselectivity, regioselectivity …

The biotechnological potential of the Chloroflexota phylum

A Freches, JC Fradinho - Applied and Environmental …, 2024 - Am Soc Microbiol
In the next decades, the increasing material and energetic demand to support population
growth and higher standards of living will amplify the current pressures on ecosystems and …

Ancestral sequence reconstruction enhances gene mining efforts for industrial ene reductases by expanding enzyme panels with thermostable catalysts

J Livada, AM Vargas, CA Martinez, RD Lewis - ACS Catalysis, 2023 - ACS Publications
Ancestral sequence reconstruction (ASR) has been used to produce enzymes that exhibit
enhanced stability, activity, and promiscuity compared to wild-type (WT) enzymes. In this …

Structural and functional characterization of a new thermophilic-like OYE from Aspergillus flavus

N Li, Y Wang, Y Meng, Y Lv, S Zhang, S Wei… - Applied Microbiology …, 2024 - Springer
Old yellow enzymes (OYEs) have been proven as powerful biocatalysts for the asymmetric
reduction of activated alkenes. Fungi appear to be valuable sources of OYEs, but most of the …

Purification and biochemical characterization of a novel ene- reductase from Kazachstania exigua HSC6 for dihydro-β-ionone from β-ionone

Z Long, K Li, Y Xue, Y Sun, J Li, Z Su, J Sun, Q Liu… - Biotechnology …, 2023 - Springer
Purpose We purified and characterized a novel ene-reductase (KaDBR1) from Kazachstania
exigua HSC6 for the synthesis of dihydro-β-ionone from β-ionone. Methods KaDBR1 was …

The family keeps on growing: four novel fungal OYEs characterized

MS Robescu, G Loprete, M Gasparotto… - International Journal of …, 2022 - mdpi.com
Aiming at expanding the portfolio of Old Yellow Enzymes (OYEs), which have been
systematically studied to be employed in the chemical and pharmaceutical industries as …

Identification of Five Robust Novel Ene-Reductases from Thermophilic Fungi

PH Damada, MW Fraaije - Catalysts, 2024 - research.rug.nl
Ene-reductases (ERs) are enzymes known for catalyzing the asymmetric hydrogenation of
activated alkenes. Among these, old yellow enzyme (OYE) ERs have been the most …

Immobilization of Ene Reductase in Polyvinyl Alcohol Hydrogel

D Alagöz, NE Varan, A Toprak, SS Tükel, D Yildirim - The Protein Journal, 2022 - Springer
In this study, ene reductase (ER) was entrapped in polyvinyl alcohol hydrogel, adsorbed on
montmorillonite and immobilized covalently on glutaraldehyde activated 3-aminopropyl …

[HTML][HTML] Comparative study of Thermotolerant Hexavalent Cr Bioremediating Bacteria from Dharavi in India

V Manoranjitham, JR Chunduri - … Biotechnology Research Asia, 2024 - biotech-asia.org
The Indian leather industry, crucial for the economy, relies on chrome tanning, using 69,000
tons of chromium annually, with 39% ending up as hazardous waste. The non …

[引用][C] Biocatalytic Reductions (C–O, C–N, C–C)

RD Lewis, J Livada, CA Martinez - 2024 - Elsevier