Advances in genome editing through control of DNA repair pathways
Eukaryotic cells deploy overlapping repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …
Advancements in genome editing take advantage of these pathways to produce permanent …
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …
comprise a powerful class of tools that are redefining the boundaries of biological research …
Precise cut-and-paste DNA insertion using engineered type VK CRISPR-associated transposases
CJ Tou, B Orr, BP Kleinstiver - Nature Biotechnology, 2023 - nature.com
CRISPR-associated transposases (CASTs) enable recombination-independent, multi-
kilobase DNA insertions at RNA-programmed genomic locations. However, the utility of type …
kilobase DNA insertions at RNA-programmed genomic locations. However, the utility of type …
A guide to genome engineering with programmable nucleases
H Kim, JS Kim - Nature Reviews Genetics, 2014 - nature.com
Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
Genome-editing technologies: principles and applications
Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …
Genome engineering with targetable nucleases
D Carroll - Annual review of biochemistry, 2014 - annualreviews.org
Current technology enables the production of highly specific genome modifications with
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …
Poly (ADP-ribose) polymerases in double-strand break repair: focus on PARP1, PARP2 and PARP3
Abstract Poly (ADP-ribosyl) ation (PARylation) is a post-translational modification of proteins
catalysed by Poly (ADP-ribose) polymerases (PARP). A wealth of recent advances in the …
catalysed by Poly (ADP-ribose) polymerases (PARP). A wealth of recent advances in the …
Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells
A Tomita, H Sasanuma, T Owa, Y Nakazawa… - Nature …, 2023 - nature.com
CRISPR/Cas9-mediated gene editing has great potential utility for treating genetic diseases.
However, its therapeutic applications are limited by unintended genomic alterations arising …
However, its therapeutic applications are limited by unintended genomic alterations arising …
Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy
G Silva, L Poirot, R Galetto, J Smith… - Current gene …, 2011 - ingentaconnect.com
The importance of safer approaches for gene therapy has been underscored by a series of
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …
The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair
M Jasin, JE Haber - DNA repair, 2016 - Elsevier
DNA double-strand breaks (DSBs) are dangerous lesions that if not properly repaired can
lead to genomic change or cell death. Organisms have developed several pathways and …
lead to genomic change or cell death. Organisms have developed several pathways and …