[HTML][HTML] A universal method for high-quality RNA extraction from plant tissues rich in starch, proteins and fiber

AR Vennapusa, IM Somayanda, CJ Doherty… - Scientific reports, 2020 - nature.com
Using existing protocols, RNA extracted from seeds rich in starch often results in poor quality
RNA, making it inappropriate for downstream applications. Though some methods are …

[HTML][HTML] Proteomics, physiological, and biochemical analysis of cross tolerance mechanisms in response to heat and water stresses in soybean

R Katam, S Shokri, N Murthy, SK Singh, P Suravajhala… - Plos one, 2020 - journals.plos.org
Water stress (WS) and heat stress (HS) have a negative effect on soybean plant growth and
crop productivity. Changes in the physiological characteristics, proteome, and specific …

[HTML][HTML] Isolation of total RNA from tissues rich in polyphenols and polysaccharides of mangrove plants

JA Rubio-Piña, O Zapata-Pérez - Electronic journal of Biotechnology, 2011 - SciELO Chile
RNA extraction from mangrove tissues can be difficult due to the presence of polyphenolic
and polysaccharide compounds upon cell disruption. Besides, a successful RNA isolation …

Suppression subtractive hybridization versus next-generation sequencing in plant genetic engineering: challenges and perspectives

M Sahebi, MM Hanafi, P Azizi, A Hakim… - Molecular …, 2015 - Springer
Suppression subtractive hybridization (SSH) is an effective method to identify different genes
with different expression levels involved in a variety of biological processes. This method …

[HTML][HTML] Global transcriptional analysis suggests Lasiodiplodia theobromae pathogenicity factors involved in modulation of grapevine defensive response

M Paolinelli-Alfonso, JM Villalobos-Escobedo… - BMC genomics, 2016 - Springer
Background Lasiodiplodia theobromae is a fungus of the Botryosphaeriaceae that causes
grapevine vascular disease, especially in regions with hot climates. Fungi in this group often …

Comparative leaf proteomics of drought-tolerant and-susceptible peanut in response to water stress

R Katam, K Sakata, P Suravajhala, T Pechan… - Journal of …, 2016 - Elsevier
Water stress (WS) predisposes peanut plants to fungal infection resulting in pre-harvest
aflatoxin contamination. Major changes during water stress including oxidative stress, lead …

[HTML][HTML] RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics

V Thole, JE Bassard, R Ramírez-González, M Trick… - BMC genomics, 2019 - Springer
Background Flavonoids are produced in all flowering plants in a wide range of tissues
including in berry fruits. These compounds are of considerable interest for their biological …

Differential induction of antioxidant stilbenoids in hairy roots of Vitis rotundifolia treated with methyl jasmonate and hydrogen peroxide

C Nopo-Olazabal, J Condori, L Nopo-Olazabal… - Plant Physiology and …, 2014 - Elsevier
Stilbenoids are polyphenolic phytoalexins that exhibit potential health applications in
humans. Hairy root cultures of muscadine grape (Vitis rotundifolia Michx.) were used to …

[HTML][HTML] Rapid and reliable method of high-quality RNA extraction from diverse plants

SK Sah, G Kaur, A Kaur - American Journal of Plant Sciences, 2014 - scirp.org
The isolation of high quality RNA is a crucial technique in plant molecular biology. The
quality of RNA determines the reliability of downstream process like real time PCR. In this …

[HTML][HTML] Standardization of an efficient protocol for isolation of RNA from Cuminum cyminum

P Kanani, YM Shukla, AR Modi, N Subhash… - Journal of King Saud …, 2019 - Elsevier
Cumin is one of the oldest seed species and second most popular after black pepper. Till
date molecular work into cumin has been principally related to studying diversity based on …