Visualization of endogenous G proteins on endosomes and other organelles

W Jang, K Senarath, G Feinberg, S Lu, NA Lambert - Elife, 2024 - elifesciences.org
Classical G-protein-coupled receptor (GPCR) signaling takes place in response to
extracellular stimuli and involves receptors and heterotrimeric G proteins located at the …

Advances in spatial proteomics: Mapping proteome architecture from protein complexes to subcellular localizations

LM Breckels, C Hutchings, KD Ingole, S Kim… - Cell Chemical …, 2024 - cell.com
Proteins are responsible for most intracellular functions, which they perform as part of higher-
order molecular complexes, located within defined subcellular niches. Localization is both …

AI: A transformative opportunity in cell biology

A Carr, J Cool, T Karaletsos, D Li, AR Lowe… - Molecular Biology of …, 2024 - molbiolcell.org
The success of artificial intelligence (AI) algorithms in predicting protein structure and more
recently, protein interactions, demonstrates the power and potential of machine learning and …

Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells

IE Ivanov, E Hirata-Miyasaki, T Chandler… - PNAS …, 2024 - academic.oup.com
High-throughput dynamic imaging of cells and organelles is essential for understanding
complex cellular responses. We report Mantis, a high-throughput 4D microscope that …

[HTML][HTML] CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly

AJ Mathiowetz, ES Meymand, KK Deol, G Parlakgül… - BioRxiv, 2024 - pmc.ncbi.nlm.nih.gov
Imbalances in lipid storage and secretion lead to the accumulation of hepatocyte lipid
droplets (LDs)(ie, hepatic steatosis). Our understanding of the mechanisms that govern the …

Identification and characterization of a human MORC2 DNA binding region that is required for gene silencing

NL Fendler, J Ly, L Welp, D Lu, F Schulte… - Nucleic Acids …, 2024 - academic.oup.com
The eukaryotic microrchidia (MORC) protein family are DNA g yrase, H sp90, histidine k
inase, Mut L (GHKL)-type ATPases involved in gene expression regulation and chromatin …

Adaptations of membrane trafficking in cancer and tumorigenesis

E Evergren, IG Mills, G Kennedy - Journal of Cell Science, 2024 - journals.biologists.com
Membrane trafficking, a fundamental cellular process encompassing the transport of
molecules to specific organelles, endocytosis at the plasma membrane and protein …

Annotating CryoET Volumes: A Machine Learning Challenge

A Peck, Y Yu, J Schwartz, A Cheng, UH Ermel… - bioRxiv, 2024 - biorxiv.org
Cryo-electron tomography (cryoET) has emerged as a powerful structural biology tool for
understanding protein complexes in their native cellular environments. Presently, 3D …

Automated segmentation of soft X-ray tomography: native cellular structure with sub-micron resolution at high throughput for whole-cell quantitative imaging in yeast

JHF Chen, M Mirvis, A Ekman, B Vanslembrouck… - bioRxiv, 2024 - biorxiv.org
Soft X-ray tomography (SXT) is an invaluable tool for quantitatively analyzing cellular
structures at sub-optical isotropic resolution. However, it has traditionally depended on …

Finding order in chaos: Dissecting single-cell heterogeneity in space and time

C Gnann - 2024 - diva-portal.org
The cell is the smallest unit of life and contains DNA, RNA, proteins and a variety of other
macromolecules. In recent years, technological advances in the field of single cell biology …