Powerful methods now allow the imaging of specific mRNAs in living cells. These methods
enlist fluorescent proteins to illuminate mRNAs, use labeled oligonucleotide probes and …

The dynamics governing gene regulation have an important role in determining the
phenotype of a cell or organism. From processing extracellular signals to generating internal …

Because RNA lacks strong intrinsic fluorescence, it has proven challenging to track RNA
molecules in real time. To address this problem and to allow the purification of fluorescently …

Live-cell imaging of mRNA yields important insights into gene expression, but it has
generally been limited to the labeling of one RNA species and has never been used to count …

Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease primarily
affecting motor neurons. Mutations in the gene encoding TDP-43 cause some forms of the …

Commonly used techniques for analyzing gene expression, such as polymerase chain
reaction (PCR), microarrays, and in situ hybridization, have proven invaluable in …

High-throughput gene expression screens provide a quantitative picture of the average
expression signature of biological samples. However, the analysis of spatial gene …

Optical imaging of single biomolecules and complexes in living cells provides a useful
window into cellular processes. However, the three-dimensional dynamics of most important …

Targeted mRNA localization is a likely determinant of localized protein synthesis. To
investigate whether mRNAs encoding mitochondrial proteins (mMPs) localize to …

The faithful assembly of protein complexes in space and time is a hallmark of cellular
homeostasis. Complex assembly might be seeded already during translation, if interacting …