Cytochrome P450s (P450s) comprise one of the largest known protein families. They occur
in every kingdom of life and catalyze essential reactions, such as carbon source …

Membrane proteins are present in a wide array of cellular processes from primary and
secondary metabolite synthesis to electron transport and single carbon metabolism. A key …

By exploiting versatile P450 enzymes, whole‐cell biocatalysis can be performed to
synthesize valuable compounds in Escherichia coli. However, the insufficient supply of …

Pseudomonas aeruginosa is an opportunistic bacterium that causes life-threatening
infections in immunocompromised patients. In infection, it uses heme as a primary iron …

Abstract Cytochrome P450 (CYP) heme‐thiolate monooxygenases catalyze the
hydroxylation of the C− H bonds of organic molecules. This reaction is initiated by a ferryl …

Exchanging the native iron of heme for other metals yields artificial metalloproteins with new
properties for spectroscopic studies and biocatalysis. Recently, we reported a method for the …

Lignin is Nature's major source of aromatic chemistry and is by many seen as the green
entry-point alternative to the fossil-based chemical industry. Due to its chemically recalcitrant …

The production of aldehydes, highly reactive and toxic chemicals, brings specific challenges
to biocatalytic processes. Absence of natural accumulation of aldehydes in microorganisms …

Recombinant production of heme proteins in Escherichia coli is often limited by the
availability of heme in the host. Therefore, several methods, including the reconstitution of …

Background Enzymes from thermophiles are of great interest for research and
bioengineering due to their stability and efficiency. Thermophilic expression hosts such as …