The advent of genome editing has transformed the therapeutic landscape for several
debilitating diseases, and the clinical outlook for gene therapeutics has never been more …

The targeted and efficiency-oriented delivery of (therapeutic) nucleic acids raises hope for
successful gene therapy, ie, for the local and individual treatment of acquired and inherited …

In non-viral gene therapy, cationic polymers and lipids are frequently used to encapsulate
macromolecular therapeutics into nanoparticles. During their journey to deliver the cargo to …

Polycations such as polyethylenimine (PEI) are used in many novel nonviral vector designs
and there are continuous efforts to increase our mechanistic understanding of their …

Background The relatively high transfection efficiency of polyethylenimine (PEI) vectors has
been hypothesized to be due to their ability to avoid trafficking to degradative lysosomes …

Gene therapy has become a promising strategy for the treatment of many inheritable or
acquired diseases that are currently considered incurable. Non-viral vectors have attracted …

Hyperbranched polymers (HBPs) are highly branched macromolecules with a three‐
dimensional dendritic architecture. Due to their unique topological structure and interesting …

We investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine
and K+ depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization …

Gene therapy has long sought to treat disease by delivering therapeutic genes to diseased
cells. However, its widespread clinical use has faltered in the face of several challenges …

Synthetic polymer based Polyfectins (cationic polymer-DNA complex) have received
intensive scientific research as they can potentially circumvent problems associated with …