Transfection is a modern and powerful method used to insert foreign nucleic acids into
eukaryotic cells. The ability to modify host cells' genetic content enables the broad …

To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

The targeted deletion, replacement, integration or inversion of genomic sequences could be
used to study or treat human genetic diseases, but existing methods typically require double …

Large serine recombinases (LSRs) are DNA integrases that facilitate the site-specific
integration of mobile genetic elements into bacterial genomes. Only a few LSRs, such as …

Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of
its long history in industrial fermentations. However, until recently, most synthetic biology …

The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

DNA assembly is a key part of constructing gene expression systems and even whole
chromosomes. In the past decade, a plethora of powerful new DNA assembly methods …

Multiplex genetic assays can simultaneously test thousands of genetic variants for a property
of interest. However, limitations of existing multiplex assay methods in cultured mammalian …

Engineering mammalian cell lines that stably express many transgenes requires the precise
insertion of large amounts of heterologous DNA into well-characterized genomic loci, but …

Chemical synthesis is a well established route for producing many chemicals on a large
scale, but some drawbacks still exist in this process, such as unstable intermediates …