Multiplexed CRISPR technologies for gene editing and transcriptional regulation

NS McCarty, AE Graham, L Studená… - Nature …, 2020 - nature.com
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology

M Hesami, M Pepe, A Baiton, AMP Jones - Biotechnology Advances, 2023 - Elsevier
For centuries, cannabis has been a rich source of fibrous, pharmaceutical, and recreational
ingredients. Phytocannabinoids are the most important and well-known class of cannabis …

Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts

P Patra, M Das, P Kundu, A Ghosh - Biotechnology Advances, 2021 - Elsevier
Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon
sources is currently an advancing area in industrial research. The model yeast …

Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories …

C Madzak - Journal of Fungi, 2021 - mdpi.com
Among non-conventional yeasts of industrial interest, the dimorphic oleaginous yeast
Yarrowia lipolytica appears as one of the most attractive for a large range of white …

Metabolic engineering in the host Yarrowia lipolytica

AM Abdel-Mawgoud, KA Markham, CM Palmer… - Metabolic …, 2018 - Elsevier
The nonconventional, oleaginous yeast, Yarrowia lipolytica is rapidly emerging as a
valuable host for the production of a variety of both lipid and nonlipid chemical products …

[HTML][HTML] Synthetic biology tools for engineering Yarrowia lipolytica

M Larroudé, T Rossignol, JM Nicaud… - Biotechnology …, 2018 - Elsevier
The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It
naturally produces certain compounds of interest but can also artificially generate non-native …

CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications

P Cai, J Gao, Y Zhou - Microbial cell factories, 2019 - Springer
Non-conventional yeasts are playing important roles as cell factories for bioproduction of
biofuels, food additives and proteins with outstanding natural characteristics. However, the …

Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis

S Thorwall, C Schwartz, JW Chartron… - Nature chemical …, 2020 - nature.com
Microbial chemical production is a rapidly growing industry, with much of the growth fueled
by advances in synthetic biology. New approaches have enabled rapid strain engineering …

CRISPR/dCas9-based systems: mechanisms and applications in plant sciences

CKS Karlson, SN Mohd-Noor, N Nolte, BC Tan - Plants, 2021 - mdpi.com
RNA-guided genomic transcriptional regulation tools, namely clustered regularly
interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene …

Employment of the CRISPR/Cas9 system to improve cellulase production in Trichoderma reesei

S Pant, P Nag, A Ghati, D Chakraborty… - Biotechnology …, 2022 - Elsevier
Trichoderma reesei has been explored intensively in the laboratory and on an industrial
scale for its highly potent cellulase secretion machinery since its characterization over 70 …