Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units

A Bielanski, G Vajta - Human reproduction, 2009 - academic.oup.com
Cryopreservation of sperm, embryos and, more recently, oocytes plays an important and
increasing role in assisted reproduction, due to improvements of old, and introduction of new …

A review of the risk of contamination of semen and embryos during cryopreservation and measures to limit cross-contamination during banking to prevent disease …

A Bielanski - Theriogenology, 2012 - Elsevier
This review summarizes pertinent data and opinions regarding the potential hazard of
disease transmission through cryopreserved and banked embryos in liquid nitrogen (LN) …

Direct transfer of frozen-thawed bovine embryos

SA Voelkel, YX Hu - Theriogenology, 1992 - Elsevier
The ability to transfer frozen embryos directly to recipient females after thawing would be a
convenience in embryo transfer and would facilitate marketing embryos produced in large …

[HTML][HTML] The principal variables of cryopreservation: solutions, temperatures, and rate changes

SP Leibo, TB Pool - Fertility and sterility, 2011 - Elsevier
OBJECTIVE: To describe several fundamental variables that influence ultimate survival of
oocytes and embryos when they are cryopreserved. DESIGN: The literature describing …

Cryopreservation of ovine embryos: slow freezing and vitrification

AG Martinez, M Matkovic - Theriogenology, 1998 - Elsevier
Different methods for the cryopreservation of ovine embryos were evaluated in vitro (survival
upon culture in vitro) and in vivo (pregnancy and lambing rates after transfer in field …

Effect of some permeating cryoprotectants on CASA motility results in cryopreserved bull spermatozoa

MM Awad - Animal reproduction science, 2011 - Elsevier
Computer-assisted sperm analyzers (CASA) have become the standard tool for evaluating
sperm motility because they provide objective results for thousands of mammalian …

Ultrastructural characteristics of fresh and frozen-thawed ovine embryos using two cryoprotectants

MJ Cocero, SM Díaz de la Espina… - Biology of …, 2002 - academic.oup.com
Cryopreservation of sheep embryos with ethylene glycol as a protectant appears to be more
effective than glycerol, particularly at the morula stage, as has been demonstrated on the …

Methanol as a cryoprotectant for equine embryos

LD Bass, DJ Denniston, LJ Maclellan, PM McCue… - Theriogenology, 2004 - Elsevier
Equine embryos (n= 43) were recovered nonsurgically 7–8 days after ovulation and
randomly assigned to be cryopreserved in one of two cryoprotectants: 48%(15M) methanol …

Sheep embryo cryopreservation by vitrification and conventional freezing

P De Paz, AJ Sanchez, JG Fernandez, M Carbajo… - Theriogenology, 1994 - Elsevier
The survival of ovine embryos (morulae and blastocysts) either frozen by a conventional
method or vitrified was investigated in culture. In Experiment I, embryos were vitrified using a …

Permeation and toxicity of ethylene glycol and methanol in larvae of Anopheles gambiae

XH Liu, H Pan, P Mazur - Journal of experimental biology, 2003 - journals.biologists.com
In this study, we applied proton NMR to measure the permeation of two cryoprotective
agents (CPAs), ethylene glycol (EG) and methanol, into 1st instar Anopheles larvae …