Methods for transfer of exogenous DNA into cells are essential for genetics and molecular
biology, and the lack of effective methods hampers research on many different species of …

The ability to introduce individual molecules of plasmid DNA into cells by transformation has
been of central importance to the recent rapid advancement of plasmid biology and to the …

In a previous communication, we reported a new method of synthesis of stable metallic
copper nanoparticles (Cu-NPs), which had high potency for bacterial cell filamentation and …

The dissemination of antibiotic resistance mediated by horizontal transfer of antibiotic
resistance genes (ARGs) is exacerbating the global antibiotic crisis. Currently, little is known …

CaCl 2 treatment followed by heat shock is the most common method for artificial
transformation. Here, the cells were transformed using CaCl 2 treatment either with heat …

Research on the biocathode-based bioelectrochemical system (BES) has attracted
extensive attention because of its ability to increase the electricity-driven production of high …

Artificial transformation of Escherichia coli with plasmid DNA in presence of CaCl2 is a
widely used technique in recombinant DNA technology. However, exact mechanism of DNA …

Abstract Cu 2+, tetracycline (TC), and corresponding tetracycline resistance genes (TRGs)
are common micropollutants in aquaculture wastewater, which have great impact on …

The preparation of competent cell is the central step of bacteria transformation and has a
great impact on transformation efficiency of nucleic acid. The aim of the research was to …

Diabetes is a menacing problem, particularly to inhabitants of groundwater arsenic
contaminated areas needing new medical approaches. This study examines if PLGA loaded …