The emergence of phase separation as an organizing principle in bacteria

CA Azaldegui, AG Vecchiarelli, JS Biteen - Biophysical journal, 2021 - cell.com
Recent investigations in bacteria suggest that membraneless organelles play a crucial role
in the subcellular organization of bacterial cells. However, the biochemical functions and …

Nuclear bodies: the emerging biophysics of nucleoplasmic phases

L Zhu, CP Brangwynne - Current opinion in cell biology, 2015 - Elsevier
The cell nucleus contains a large number of membrane-less bodies that play important roles
in the spatiotemporal regulation of gene expression. Recent work suggests that low …

Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus

X Wang, HB Brandão, TBK Le, MT Laub, DZ Rudner - Science, 2017 - science.org
Structural maintenance of chromosomes (SMC) complexes play critical roles in chromosome
dynamics in virtually all organisms, but how they function remains poorly understood. In the …

[HTML][HTML] ParB-type DNA segregation proteins are CTP-dependent molecular switches

M Osorio-Valeriano, F Altegoer, W Steinchen, S Urban… - Cell, 2019 - cell.com
During cell division, newly replicated DNA is actively segregated to the daughter cells. In
most bacteria, this process involves the DNA-binding protein ParB, which condenses the …

Dynamic ParB–DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation

M Tišma, R Janissen, H Antar… - Nucleic acids …, 2023 - academic.oup.com
In most bacteria, chromosome segregation is driven by the ParAB S system where the
CTPase protein ParB loads at the parS site to trigger the formation of a large partition …

ATP-driven separation of liquid phase condensates in bacteria

B Guilhas, JC Walter, J Rech, G David, NO Walliser… - Molecular Cell, 2020 - cell.com
Liquid-liquid phase-separated (LLPS) states are key to compartmentalizing components in
the absence of membranes; however, it is unclear whether LLPS condensates are actively …

Nucleic acids modulate liquidity and dynamics of artificial membraneless organelles

J Liu, F Zhorabek, Y Chau - ACS Macro Letters, 2022 - ACS Publications
Liquid–liquid phase separation (LLPS) emerges as a fundamental underlying mechanism
for the biological organization, especially the formation of membraneless organelles (MLOs) …

ParB proteins can bypass DNA-bound roadblocks via dimer-dimer recruitment

M Tišma, M Panoukidou, H Antar, YM Soh, R Barth… - Science …, 2022 - science.org
The ParAB S system is essential for prokaryotic chromosome segregation. After loading at
parS on the genome, ParB (partition protein B) proteins rapidly redistribute to distances of …

Bacterial chromosome segregation by the ParABS system

ASB Jalal, TBK Le - Open biology, 2020 - royalsocietypublishing.org
Proper chromosome segregation during cell division is essential in all domains of life. In the
majority of bacterial species, faithful chromosome segregation is mediated by the tripartite …

ParB spreading requires DNA bridging

TGW Graham, X Wang, D Song, CM Etson… - Genes & …, 2014 - genesdev.cshlp.org
The parABS system is a widely employed mechanism for plasmid partitioning and
chromosome segregation in bacteria. ParB binds to parS sites on plasmids and …