Technical aspects and recommendations for single-cell qPCR

A Ståhlberg, M Kubista - Molecular aspects of medicine, 2018 - Elsevier
Single cells are basic physiological and biological units that can function individually as well
as in groups in tissues and organs. It is central to identify, characterize and profile single …

Practical data handling pipeline improves performance of qPCR-based circulating miRNA measurements

MWJ De Ronde, JM Ruijter, D Lanfear, A Bayes-Genis… - Rna, 2017 - rnajournal.cshlp.org
Since numerous miRNAs have been shown to be present in circulation, these so-called
circulating miRNAs have emerged as potential biomarkers for disease. However, results of …

Comparison of remote sensing time-series smoothing methods for grassland spring phenology extraction on the Qinghai–Tibetan Plateau

N Li, P Zhan, Y Pan, X Zhu, M Li, D Zhang - Remote Sensing, 2020 - mdpi.com
Accurate evaluation of start of season (SOS) changes is essential to assess the ecosystem's
response to climate change. Smoothing method is an understudied factor that can lead to …

Dynamics and drivers of vegetation phenology in three-river headwaters region based on the Google Earth engine

J Wang, H Sun, J Xiong, D He, W Cheng, C Ye… - Remote Sensing, 2021 - mdpi.com
Phenology shifts over time are known as the canary in the mine when studying the response
of terrestrial ecosystems to climate change. Plant phenology is a key factor controlling the …

Towards identification of postharvest fruit quality transcriptomic markers in Malus domestica

JA Hadish, HL Hargarten, H Zhang, JP Mattheis… - Plos one, 2024 - journals.plos.org
Gene expression is highly impacted by the environment and can be reflective of past events
that affected developmental processes. It is therefore expected that gene expression can …

Development of a portable electrochemical loop mediated isothermal amplification (LAMP) device for detection of hepatitis B virus

NY Jayanath, LT Nguyen, TT Vu, L Dai Tran - RSC advances, 2018 - pubs.rsc.org
The objective of this study was to develop a simple, inexpensive prototype device for rapid
detection of hepatitis B virus (HBV). The device was able to simultaneously amplify, detect …

The AT-hook is an evolutionarily conserved auto-regulatory domain of SWI/SNF required for cell lineage priming

D Saha, S Hailu, A Hada, J Lee, J Luo… - Nature …, 2023 - nature.com
Abstract The SWI/SNF ATP-dependent chromatin remodeler is a master regulator of the
epigenome, controlling pluripotency and differentiation. Towards the C-terminus of the …

Removal of artifact bias from qPCR results using DNA melting curve analysis

JM Ruijter, A Ruiz‐Villalba… - The FASEB …, 2019 - Wiley Online Library
Quantitative PCR (qPCR) allows the precise measurement of DNA concentrations and is
generally considered to be straightforward and trouble free. However, analyses using …

Data treatment methods for real-time colorimetric loop-mediated isothermal amplification reactions

EYS Kim, LM Imamura, B Winkert Raddatz… - Scientific Reports, 2023 - nature.com
With the SARS-CoV-2 pandemic and the need for affordable and rapid mass testing,
colorimetric isothermal amplification reactions such as Loop-Mediated Isothermal …

chipPCR: an R package to pre-process raw data of amplification curves

S Rödiger, M Burdukiewicz, P Schierack - Bioinformatics, 2015 - academic.oup.com
Motivation: Both the quantitative real-time polymerase chain reaction (qPCR) and
quantitative isothermal amplification (qIA) are standard methods for nucleic acid …