Mechanisms and regulation of DNA replication initiation in eukaryotes
MW Parker, MR Botchan, JM Berger - Critical reviews in …, 2017 - Taylor & Francis
Cellular DNA replication is initiated through the action of multiprotein complexes that
recognize replication start sites in the chromosome (termed origins) and facilitate duplex …
recognize replication start sites in the chromosome (termed origins) and facilitate duplex …
Single-molecule views of protein movement on single-stranded DNA
The advent of new technologies allowing the study of single biological molecules continues
to have a major impact on studies of interacting systems as well as enzyme reactions. These …
to have a major impact on studies of interacting systems as well as enzyme reactions. These …
Structure of the eukaryotic MCM complex at 3.8 Å
DNA replication in eukaryotes is strictly regulated by several mechanisms. A central step in
this replication is the assembly of the heterohexameric minichromosome maintenance …
this replication is the assembly of the heterohexameric minichromosome maintenance …
The minichromosome maintenance replicative helicase
SD Bell, MR Botchan - Cold Spring Harbor perspectives …, 2013 - cshperspectives.cshlp.org
The eukaryotic replicative helicase, the minichromosome maintenance (MCM) complex, is
composed of six distinct, but related, subunits MCM (2–7). The relationship between the …
composed of six distinct, but related, subunits MCM (2–7). The relationship between the …
Mechanisms of hexameric helicases
AJ Fernandez, JM Berger - Critical reviews in biochemistry and …, 2021 - Taylor & Francis
Ring-shaped hexameric helicases are essential motor proteins that separate duplex nucleic
acid strands for DNA replication, recombination, and transcriptional regulation. Two …
acid strands for DNA replication, recombination, and transcriptional regulation. Two …
The DNA replication fork suppresses CMG unloading from chromatin before termination
When converging replication forks meet during replication termination, the CMG (Cdc45–
MCM2–7–GINS) helicase is polyubiquitylated by CRL2 Lrr1 and unloaded from chromatin …
MCM2–7–GINS) helicase is polyubiquitylated by CRL2 Lrr1 and unloaded from chromatin …
Cdc45 (cell division cycle protein 45) guards the gate of the Eukaryote Replisome helicase stabilizing leading strand engagement
T Petojevic, JJ Pesavento, A Costa… - Proceedings of the …, 2015 - National Acad Sciences
DNA replication licensing is now understood to be the pathway that leads to the assembly of
double hexamers of minichromosome maintenance (Mcm2–7) at origin sites. Cell division …
double hexamers of minichromosome maintenance (Mcm2–7) at origin sites. Cell division …
Dynamics of the eukaryotic replicative helicase at lagging-strand protein barriers support the steric exclusion model
Progression of DNA replication depends on the ability of the replisome complex to overcome
nucleoprotein barriers. During eukaryotic replication, the CMG helicase translocates along …
nucleoprotein barriers. During eukaryotic replication, the CMG helicase translocates along …
Translocation and stability of replicative DNA helicases upon encountering DNA-protein cross-links
T Nakano, M Miyamoto-Matsubara… - Journal of Biological …, 2013 - ASBMB
DNA-protein cross-links (DPCs) are formed when cells are exposed to various DNA-
damaging agents. Because DPCs are extremely large, steric hindrance conferred by DPCs …
damaging agents. Because DPCs are extremely large, steric hindrance conferred by DPCs …
Action of CMG with strand-specific DNA blocks supports an internal unwinding mode for the eukaryotic replicative helicase
L Langston, M O'Donnell - Elife, 2017 - elifesciences.org
Replicative helicases are ring-shaped hexamers that encircle DNA for duplex unwinding.
The currently accepted view of hexameric helicase function is by steric exclusion, where the …
The currently accepted view of hexameric helicase function is by steric exclusion, where the …