Proteins are molecular machines whose function depends on their ability to achieve
complex folds with precisely defined structural and dynamic properties. The rational design …

De novo protein design has proven to be a powerful tool for understanding protein folding,
structure, and function. In this Account, we highlight aspects of our research on the design of …

Import of proteins into mitochondria occurs by coordinated actions of preprotein translocases
in the outer and inner membranes. Tim9 and Tim10 are translocase components of the …

Combinatorial experiments provide new ways to probe the determinants of protein folding
and to identify novel folding amino acid sequences. These types of experiments, however …

The understanding at the atomic level of how and why proteins fold is crucial to the
understanding of the life processes and to our ability of designing novel proteins with …

We present the results of sequence design on our off-lattice minimalist model in which no
specification of native-state tertiary contacts is needed. We start with a sequence that adopts …

Several studies have demonstrated that proteins incorporating fluorinated analogues of
hydrophobic amino acids such as leucine and valine into their hydrophobic cores exhibit …

The progress achieved by several groups in the field of computational protein design shows
that successful design methods include two major features: efficient algorithms to deal with …

Protein design tests our understanding of protein stability and structure. Successful design
methods should allow the exploration of sequence space not found in nature. However …

De novo sequence design of foldable proteins provides a way of investigating principles of
protein architecture. We performed fully automated sequence design for a target structure …