Growth-coupled high throughput selection for directed enzyme evolution
Z Li, Y Deng, GY Yang - Biotechnology Advances, 2023 - Elsevier
Directed enzyme evolution has revolutionized the rapid development of enzymes with
desired properties. However, the lack of a high-throughput method to identify the most …
desired properties. However, the lack of a high-throughput method to identify the most …
Biosynthesis and degradation of sulfur modifications in tRNAs
N Shigi - International Journal of Molecular Sciences, 2021 - mdpi.com
Various sulfur-containing biomolecules include iron–sulfur clusters that act as cofactors for
enzymes, sulfur-containing vitamins such as thiamin, and sulfur-modified nucleosides in …
enzymes, sulfur-containing vitamins such as thiamin, and sulfur-modified nucleosides in …
Cytidine deaminases catalyze the conversion of N(S,O)4-substituted pyrimidine nucleosides
N Urbelienė, M Tiškus, G Tamulaitienė… - Science …, 2023 - science.org
Cytidine deaminases (CDAs) catalyze the hydrolytic deamination of cytidine and 2′-
deoxycytidine to uridine and 2′-deoxyuridine. Here, we report that prokaryotic homo …
deoxycytidine to uridine and 2′-deoxyuridine. Here, we report that prokaryotic homo …
TudS desulfidases recycle 4-thiouridine-5'-monophosphate at a catalytic [4Fe-4S] cluster
J Fuchs, R Jamontas, MH Hoock, J Oltmanns… - Communications …, 2023 - nature.com
In all domains of life, transfer RNAs (tRNAs) contain post-transcriptionally sulfur-modified
nucleosides such as 2-and 4-thiouridine. We have previously reported that a recombinant …
nucleosides such as 2-and 4-thiouridine. We have previously reported that a recombinant …
Recent advances in our understanding of the biosynthesis of sulfur modifications in tRNAs
N Shigi - Frontiers in Microbiology, 2018 - frontiersin.org
Sulfur is an essential element in all living organisms. In tRNA molecules, there are many
sulfur-containing nucleosides, introduced post-transcriptionally, that function to ensure …
sulfur-containing nucleosides, introduced post-transcriptionally, that function to ensure …
Structural evidence for a [4Fe‐5S] intermediate in the non‐redox desulfuration of thiouracil
J Zhou, L Pecqueur, A Aučynaitė… - Angewandte Chemie …, 2021 - Wiley Online Library
We recently discovered a [Fe‐S]‐containing protein with in vivo thiouracil desulfidase
activity, dubbed TudS. The crystal structure of TudS refined at 1.5 Å resolution is reported; it …
activity, dubbed TudS. The crystal structure of TudS refined at 1.5 Å resolution is reported; it …
YqfB protein from Escherichia coli: an atypical amidohydrolase active towards N4-acylcytosine derivatives
R Stanislauskienė, A Laurynėnas, R Rutkienė… - Scientific reports, 2020 - nature.com
Human activating signal cointegrator homology (ASCH) domain-containing proteins are
widespread and diverse but, at present, the vast majority of those proteins have no function …
widespread and diverse but, at present, the vast majority of those proteins have no function …
Effect of capsular polysaccharide phase variation on biofilm formation, motility and gene expression in Vibrio vulnificus
T Zhang, S Ji, M Zhang, F Wu, X Li, X Luo, Q Huang… - Gut Pathogens, 2024 - Springer
Vibrio vulnificus, a significant marine pathogen, undergoes opaque (Op)-translucent (Tr)
colony switching based on whether capsular polysaccharide (CPS) is produced. CPS phase …
colony switching based on whether capsular polysaccharide (CPS) is produced. CPS phase …
Discovery of bacterial deaminases that convert 5-fluoroisocytosine into 5-fluorouracil
A Aučynaitė, R Rutkienė, D Tauraitė… - Frontiers in …, 2018 - frontiersin.org
Cytosine is one of the four letters of a standard genetic code, found both in DNA and in RNA.
This heterocyclic base can be converted into uracil upon the action of the well-known …
This heterocyclic base can be converted into uracil upon the action of the well-known …
Application of the uridine auxotrophic host and synthetic nucleosides for a rapid selection of hydrolases from metagenomic libraries
N Urbelienė, S Kutanovas, R Meškienė… - Microbial …, 2019 - Wiley Online Library
A high‐throughput method (≥ 106 of clones can be analysed on a single agar plate) for the
selection of ester‐hydrolysing enzymes was developed based on the uridine auxotrophy of …
selection of ester‐hydrolysing enzymes was developed based on the uridine auxotrophy of …