Flavonoid biosynthetic pathways in plants: Versatile targets for metabolic engineering
Plants, fungi, and microorganisms are equipped with biosynthesis machinery for producing
thousands of secondary metabolites. These compounds have important functions in nature …
thousands of secondary metabolites. These compounds have important functions in nature …
The re-emergence of natural products for drug discovery in the genomics era
AL Harvey, RA Edrada-Ebel, RJ Quinn - Nature reviews drug discovery, 2015 - nature.com
Natural products have been a rich source of compounds for drug discovery. However, their
use has diminished in the past two decades, in part because of technical barriers to …
use has diminished in the past two decades, in part because of technical barriers to …
Escherichia coli as a host for metabolic engineering
Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
Microbial acetyl-CoA metabolism and metabolic engineering
Recent concerns over the sustainability of petrochemical-based processes for production of
desired chemicals have fueled research into alternative modes of production. Metabolic …
desired chemicals have fueled research into alternative modes of production. Metabolic …
Microbial production of natural and non-natural flavonoids: pathway engineering, directed evolution and systems/synthetic biology
In this review, we address recent advances made in pathway engineering, directed
evolution, and systems/synthetic biology approaches employed in the production and …
evolution, and systems/synthetic biology approaches employed in the production and …
Metabolic engineering of microorganisms for production of aromatic compounds
Metabolic engineering has been enabling development of high performance microbial
strains for the efficient production of natural and non-natural compounds from renewable …
strains for the efficient production of natural and non-natural compounds from renewable …
Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids
JA Jones, VR Vernacchio, AL Sinkoe, SM Collins… - Metabolic …, 2016 - Elsevier
Metabolic engineering and synthetic biology have enabled the use of microbial production
platforms for the renewable production of many high-value natural products. Titers and …
platforms for the renewable production of many high-value natural products. Titers and …
Evolution-guided optimization of biosynthetic pathways
Engineering biosynthetic pathways for chemical production requires extensive optimization
of the host cellular metabolic machinery. Because it is challenging to specify a priori an …
of the host cellular metabolic machinery. Because it is challenging to specify a priori an …
[HTML][HTML] Establishment of a yeast platform strain for production of p-coumaric acid through metabolic engineering of aromatic amino acid biosynthesis
A Rodriguez, KR Kildegaard, M Li, I Borodina… - Metabolic …, 2015 - Elsevier
Aromatic amino acids are precursors of numerous plant secondary metabolites with diverse
biological functions. Many of these secondary metabolites are already being used as active …
biological functions. Many of these secondary metabolites are already being used as active …
Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway
Background Heterologous expression systems based on promoters inducible with isopropyl-
β-D-1-thiogalactopyranoside (IPTG), eg, Escherichia coli BL21 (DE3) and cognate LacI Q/P …
β-D-1-thiogalactopyranoside (IPTG), eg, Escherichia coli BL21 (DE3) and cognate LacI Q/P …