CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …

Our ability to detect, image, and quantify nanoscopic objects and molecules with visible light
has undergone dramatic improvements over the past few decades. While fluorescence has …

Transposon-encoded tnpB and iscB genes encode RNA-guided DNA nucleases that
promote their own selfish spread through targeted DNA cleavage and homologous …

Recombinant adeno-associated viruses (rAAV) are used extensively as gene delivery
vectors in clinical studies, and several rAAV based treatments have already been approved …

Protein–protein interactions are involved in the regulation and function of the majority of
cellular processes. As a result, much effort has been aimed at the development of …

Prokaryotic genomes are replete with mobile genetic elements (MGE) that span a continuum
of replication autonomy. On numerous occasions during microbial evolution, diverse MGE …

Bacterial CRISPR-Cas systems provide adaptive immunity against viruses and other mobile
genome elements. During the adaptation step cells become immunized by insertion of short …

Prokaryotes are constantly coping with attacks by viruses in their natural environments and
therefore have evolved an impressive array of defense systems. Clustered Regularly …

The principal function of archaeal and bacterial CRISPR-Cas systems is antivirus adaptive
immunity. However, recent genome analyses identified a variety of derived CRISPR-Cas …

Argonaute (Ago) proteins are found in all three domains of life. The best-characterized group
is eukaryotic Argonautes (eAgos), which are the core of RNA interference. The best …