Applications of genome‐scale metabolic reconstructions

MA Oberhardt, BØ Palsson, JA Papin - Molecular systems biology, 2009 - embopress.org
The availability and utility of genome‐scale metabolic reconstructions have exploded since
the first genome‐scale reconstruction was published a decade ago. Reconstructions have …

Flux analysis and metabolomics for systematic metabolic engineering of microorganisms

Y Toya, H Shimizu - Biotechnology advances, 2013 - Elsevier
Rational engineering of metabolism is important for bio-production using microorganisms.
Metabolic design based on in silico simulations and experimental validation of the metabolic …

From zero to hero—design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production

J Becker, O Zelder, S Häfner, H Schröder… - Metabolic …, 2011 - Elsevier
Here, we describe the development of a genetically defined strain of l-lysine hyperproducing
Corynebacterium glutamicum by systems metabolic engineering of the wild type …

Engineering Corynebacterium glutamicum for high-titer biosynthesis of hyaluronic acid

F Cheng, H Yu, G Stephanopoulos - Metabolic engineering, 2019 - Elsevier
Hyaluronic acid (HA) is a member of the glycosaminoglycan family and has been widely
used in the clinical, medical, cosmetic and food industries. In this study, we constructed a …

A new genome-scale metabolic model of Corynebacterium glutamicum and its application

Y Zhang, J Cai, X Shang, B Wang, S Liu, X Chai… - Biotechnology for …, 2017 - Springer
Background Corynebacterium glutamicum is an important platform organism for industrial
biotechnology to produce amino acids, organic acids, bioplastic monomers, and biofuels …

Genome-scale modeling for metabolic engineering

E Simeonidis, ND Price - Journal of Industrial Microbiology and …, 2015 - academic.oup.com
We focus on the application of constraint-based methodologies and, more specifically, flux
balance analysis in the field of metabolic engineering, and enumerate recent developments …

Advancing metabolic engineering through systems biology of industrial microorganisms

Z Dai, J Nielsen - Current opinion in biotechnology, 2015 - Elsevier
Highlights•Systems biology enables metabolic engineering design.•Metabolic engineering
targets can be identified by systems biology tools.•Integrating systems biology and metabolic …

Applications of genome-scale metabolic network model in metabolic engineering

B Kim, WJ Kim, DI Kim, SY Lee - Journal of industrial …, 2015 - academic.oup.com
Genome-scale metabolic network model (GEM) is a fundamental framework in systems
metabolic engineering. GEM is built upon extensive experimental data and literature …

Improving putrescine production by Corynebacterium glutamicum by fine-tuning ornithine transcarbamoylase activity using a plasmid addiction system

J Schneider, D Eberhardt, VF Wendisch - Applied microbiology and …, 2012 - Springer
Corynebacterium glutamicum shows a great potential for the production of the polyamide
monomer putrescine (1, 4-diaminobutane). Previously, we constructed the putrescine …

Metabolic flux analysis and pharmaceutical production

BA Boghigian, G Seth, R Kiss, BA Pfeifer - Metabolic engineering, 2010 - Elsevier
Rational engineering of biological systems is an inherently complex process due to their
evolved nature. Metabolic engineering emerged and developed over the past 20 years as a …