Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

NMR spectroscopy is currently extensively used in binding assays for hit identification, but its
use in dissociation constant determination is more limited when compared to other …

Paramagnetic centers in biomolecules, such as specific metal ions that are bound to a
protein, affect the nuclei in their surrounding in various ways. One of these effects is the …

Protein–ligand interactions are of fundamental importance in almost all processes in living
organisms. The ligands comprise small molecules, drugs or biological macromolecules and …

A variety of nuclear magnetic resonance (NMR) applications have been developed for
structure-based drug discovery (SBDD). NMR provides many advantages over other …

Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags Page 1 1
Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags Christoph …

The presence of an unpaired electron in paramagnetic molecules generates significant
effects in NMR spectra, which can be exploited to provide restraints complementary to those …

In this review, lanthanide chelating tags and their applications to pseudocontact shift NMR
spectroscopy as well as analysis of residual dipolar couplings are covered. A complete …

Pseudocontact shifts (PCS) from paramagnetic lanthanide ions present powerful long-range
structural restraints for structural biology by NMR spectroscopy, but site-specific tagging of …

Lanthanoids are gaining popularity as paramagnetic centers for high resolution nuclear
magnetic resonance (NMR) spectroscopy. They provide valuable angular and long-distance …