RNA in extracellular vesicles

KM Kim, K Abdelmohsen, M Mustapic… - Wiley …, 2017 - Wiley Online Library
Cells release a range of membrane‐enclosed extracellular vesicles (EVs) into the
environment. Among them, exosomes and microvesicles (collectively measuring 40–1000 …

MicroRNA: function, detection, and bioanalysis

H Dong, J Lei, L Ding, Y Wen, H Ju, X Zhang - Chemical reviews, 2013 - ACS Publications
Mature microRNAs (miRNAs) are a class of evolutionally conserved, single-stranded, small
(approximately 19− 23 nucleotides), endogenously expressed, and non-protein-coding …

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA–an ISEV position paper

B Mateescu, EJK Kowal, BWM Van Balkom… - Journal of …, 2017 - Taylor & Francis
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has
been demonstrated in a multitude of different in vitro cell systems and in a variety of body …

Elucidation of Exosome Migration Across the Blood–Brain Barrier Model In Vitro

CC Chen, L Liu, F Ma, CW Wong, XE Guo… - Cellular and molecular …, 2016 - Springer
The delivery of therapeutics to the central nervous system remains a major challenge in part
due to the presence of the blood–brain barrier (BBB). Recently, cell-derived vesicles …

Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters

CP Lai, EY Kim, CE Badr, R Weissleder… - Nature …, 2015 - nature.com
Accurate spatiotemporal assessment of extracellular vesicle (EV) delivery and cargo RNA
translation requires specific and robust live-cell imaging technologies. Here we engineer …

Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate

MP Hall, J Unch, BF Binkowski, MP Valley… - ACS chemical …, 2012 - ACS Publications
Bioluminescence methodologies have been extraordinarily useful due to their high
sensitivity, broad dynamic range, and operational simplicity. These capabilities have been …

Differential fates of biomolecules delivered to target cells via extracellular vesicles

M Kanada, MH Bachmann, JW Hardy… - Proceedings of the …, 2015 - National Acad Sciences
Extracellular vesicles (EVs), specifically exosomes and microvesicles (MVs), are presumed
to play key roles in cell–cell communication via transfer of biomolecules between cells. The …

A molecular imaging primer: modalities, imaging agents, and applications

ML James, SS Gambhir - Physiological reviews, 2012 - journals.physiology.org
Molecular imaging is revolutionizing the way we study the inner workings of the human
body, diagnose diseases, approach drug design, and assess therapies. The field as a whole …

Isolation and analysis methods of extracellular vesicles (EVs)

Z Zhao, H Wijerathne, AK Godwin… - … vesicles and circulating …, 2020 - pmc.ncbi.nlm.nih.gov
Extracellular vesicles (EVs) have been recognized as an evolving biomarker within the
liquid biopsy family. While carrying both host cell proteins and different types of RNAs, EVs …

Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers

J Skog, T Würdinger, S Van Rijn, DH Meijer… - Nature cell …, 2008 - nature.com
Glioblastoma tumour cells release microvesicles (exosomes) containing mRNA, miRNA and
angiogenic proteins. These microvesicles are taken up by normal host cells, such as brain …