Terpenoids are the largest family of natural products, but prokaryotes are vastly
underrepresented in this chemical space. However, genomics supports vast untapped …

Diterpene synthase VenA is responsible for assembling venezuelaene A with a unique 5-5-
6-7 tetracyclic skeleton from geranylgeranyl pyrophosphate. VenA also demonstrates …

Eunicellane diterpenoids are a remarkable family of terpene natural products and have
been of high interest for over five decades. Widely distributed in soft corals and rare in …

Insects employ terpenoids for communication both within and between species. While
terpene synthases derived from isoprenyl diphosphate synthase have been shown to …

A grand challenge in terpene synthase (TS) enzymology is the ability to predict function from
protein sequence. Given the limited number of characterized bacterial TSs and significant …

Eunicellane diterpenoids, containing a typical 6, 10-bicycle, are bioactive compounds widely
present in marine corals, but rarely found in bacteria and plants. The intrinsic macrocycle …

Terpenoids are the largest family of natural products but are relatively rare in bacteria.
Genome mining reveals wide-spread prevalence of terpene synthases, the enzymes …

1. Introduction 2. Workflow 2.1 Extracting DNA from octocoral tissue 2.2 Extracting RNA from
octocoral tissue (Note 1) 2.3 Sequencing data preparation and TC mining 2.4 Molecular …

Marine corals, rich in diverse terpenoids, are a promising resource for drug discovery, yet
their potential is hindered by low supply. Recent reports on coral-derived terpene synthases …

Expression and purification of membrane-bound proteins remains a challenge and limits
enzymology efforts, contributing to a substantial knowledge gap in the biochemical functions …