Abstract Background The Friedman rank sum test is a widely-used nonparametric method in
computational biology. In addition to examining the overall null hypothesis of no significant …

Acanthamoebae are potentially pathogenic organisms, with a highly unique, yet still
insufficiently investigated metabolism. Many open questions can be addressed by gene …

The application of reverse transcription quantitative real-time PCR technology for the
production of gene tissue expression profiles is a widely employed approach in molecular …

The aim of this study was designed to select housekeeping genes for quantitative gene
expression analysis in yellow-feathered broilers. Twelve 3-week-old chickens were …

Accurate normalization in real-time quantitative PCR is an important step in quantification of
gene transcription pattern, in which proper application of stable reference gene (s) is crucial …

Background Melanoma differentiation associated gene 5 (MDA5) and retinoic acid-inducible
gene-I (RIG-I) selectively sense cytoplasmic viral RNA to induce an antiviral immune …

Accurate normalization of the gene expression assays, using housekeeping genes (HKGs),
is critically necessary. To do so, selection of a proper set of HKGs for a specific experiment is …

Reverse transcription quantitative real-time PCR is the most commonly used method to
detect gene expression levels. In experiments, it is often necessary to correct and …

Abstract Evaluation of reference genes for expression studies in chickens and turkeys is very
much limited and unavailable for various infectious models. In this study, eight candidate …

Background: Muscle development, egg production, and plumage colors are different
between native and broiler chickens. The study was designed to investigate why improved …