Highlights•We review recent progress in RNA synthetic biology.•New advances in synthetic
RNA regulator design have created high-performing/functioning parts.•New generation of …

Non-coding RNAs (ncRNAs) are versatile and powerful controllers of gene expression that
have been increasingly linked to cellular metabolism and phenotype. In bacteria, identified …

In bacteria and archaea, small RNAs (sRNAs) regulate complex networks through antisense
interactions with target mRNAs in trans, and riboswitches regulate gene expression in cis …

The human transcriptome contains many types of noncoding RNAs, which rival the number
of protein‐coding species. From long noncoding RNAs (lncRNAs) that are over 200 …

A key driver of synthetic biology is the development of designable genetic parts with
predictable behaviors that can be quickly implemented in complex genetic systems …

One of the long-term goals of synthetic biology is to develop designable genetic parts with
predictable behaviors that can be utilized to implement diverse cellular functions. The …

Herein we introduce a high-throughput method, INTERFACE, to reveal the capacity of
contiguous RNA nucleotides to establish in vivo intermolecular RNA interactions for the …

Synthetic small RNA regulators have emerged as a versatile tool to predictably control
bacterial gene expression. Owing to their simple design principles, small size, and highly …

Highlights•Regulatory noncoding RNAs (ncRNAs) are powerful tools in metabolic
engineering.•Adjusting expression of natural ncRNAs allows regulation of multiple …

Highlights•NGS-based RNA probing enables transcriptome-wide structurome
determination.•RNA structuromics reveals insights into RNA-structure-function …