CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
Mechanisms of DNA damage‐mediated neurotoxicity in neurodegenerative disease
G Welch, LH Tsai - EMBO reports, 2022 - embopress.org
Neurons are highly susceptible to DNA damage accumulation due to their large energy
requirements, elevated transcriptional activity, and long lifespan. While newer research has …
requirements, elevated transcriptional activity, and long lifespan. While newer research has …
CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects
Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type VA CRISPR-Cas anti-
phage immune system that can be repurposed for genome editing. Cas12a can bind and cut …
phage immune system that can be repurposed for genome editing. Cas12a can bind and cut …
Next-generation DNA damage sequencing
Cellular DNA is constantly chemically altered by exogenous and endogenous agents. As all
processes of life depend on the transmission of the genetic information, multiple biological …
processes of life depend on the transmission of the genetic information, multiple biological …
[HTML][HTML] Biomarkers of nucleic acid oxidation–A summary state-of-the-art
Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide
variety of diseases. Increasingly, interest is also focusing upon the effects of damage to the …
variety of diseases. Increasingly, interest is also focusing upon the effects of damage to the …
Advances in mapping the epigenetic modifications of 5‐methylcytosine (5mC), N6‐methyladenine (6mA), and N4‐methylcytosine (4mC)
DNA modification plays a pivotal role in regulating gene expression in cell development. As
prevalent markers on DNA, 5‐methylcytosine (5mC), N6‐methyladenine (6mA), and N4 …
prevalent markers on DNA, 5‐methylcytosine (5mC), N6‐methyladenine (6mA), and N4 …
[HTML][HTML] Towards a comprehensive view of 8-oxo-7, 8-dihydro-2'-deoxyguanosine: Highlighting the intertwined roles of DNA damage and epigenetics in genomic …
oxo-7, 8-dihydro-2′-deoxyguanosine (8-oxodG), a major product of DNA oxidation, is a pre-
mutagenic lesion which is prone to mispair, if left unrepaired, with 2′-deoxyadenosine …
mutagenic lesion which is prone to mispair, if left unrepaired, with 2′-deoxyadenosine …
Concordance of hydrogen peroxide–induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors
Oxidative DNA damage has been linked to inflammation, cancer, and aging. Here, we have
mapped two types of oxidative DNA damage, oxidized guanines produced by hydrogen …
mapped two types of oxidative DNA damage, oxidized guanines produced by hydrogen …
DNA phosphorothioate modification systems and associated phage defense systems
L Wang, Y Tang, Z Deng, S Chen - Annual Review of …, 2024 - annualreviews.org
In contrast to the well-known DNA methylation of nucleobases, DNA phosphorothioate (PT)
modification occurs in the DNA sugar-phosphate backbone. The non-bridging oxygen is …
modification occurs in the DNA sugar-phosphate backbone. The non-bridging oxygen is …
Analyzing modern biomolecules: the revolution of nucleic-acid sequencing–Review
G Dorado, S Gálvez, TE Rosales, VF Vásquez… - Biomolecules, 2021 - mdpi.com
Recent developments have revolutionized the study of biomolecules. Among them are
molecular markers, amplification and sequencing of nucleic acids. The latter is classified into …
molecular markers, amplification and sequencing of nucleic acids. The latter is classified into …