In Silico Constraint-Based Strain Optimization Methods: the Quest for Optimal Cell Factories
Shifting from chemical to biotechnological processes is one of the cornerstones of 21st
century industry. The production of a great range of chemicals via biotechnological means is …
century industry. The production of a great range of chemicals via biotechnological means is …
Computational methods in metabolic engineering for strain design
MR Long, WK Ong, JL Reed - Current opinion in biotechnology, 2015 - Elsevier
Highlights•Computational tools identify new biological routes to chemicals.•Genome-scale
models suggest genetic engineering strategies to improve production.•Methods that …
models suggest genetic engineering strategies to improve production.•Methods that …
[HTML][HTML] Growth-coupled overproduction is feasible for almost all metabolites in five major production organisms
A von Kamp, S Klamt - Nature communications, 2017 - nature.com
Computational modelling of metabolic networks has become an established procedure in
the metabolic engineering of production strains. One key principle that is frequently used to …
the metabolic engineering of production strains. One key principle that is frequently used to …
Systems biology based metabolic engineering for non-natural chemicals
Production of chemicals in microorganisms is no longer restricted to products arising from
native metabolic potential. In this review, we highlight the evolution of metabolic engineering …
native metabolic potential. In this review, we highlight the evolution of metabolic engineering …
[HTML][HTML] Co-evolution of strain design methods based on flux balance and elementary mode analysis
D Machado, MJ Herrgård - Metabolic Engineering Communications, 2015 - Elsevier
More than a decade ago, the first genome-scale metabolic models for two of the most
relevant microbes for biotechnology applications, Escherichia coli and Saccaromyces …
relevant microbes for biotechnology applications, Escherichia coli and Saccaromyces …
Metabolic assessment of E. coli as a Biofactory for commercial products
X Zhang, CJ Tervo, JL Reed - Metabolic engineering, 2016 - Elsevier
Metabolic engineering uses microorganisms to synthesize chemicals from renewable
resources. Given the thousands of known metabolites, it is unclear what valuable chemicals …
resources. Given the thousands of known metabolites, it is unclear what valuable chemicals …
[HTML][HTML] Increased 3-hydroxypropionic acid production from glycerol, by modification of central metabolism in Escherichia coli
Abstract Background 3-hydroxypropionic acid (3HP) is an important chemical precursor for
the production of bioplastics. Microbial production of 3HP from glycerol has previously been …
the production of bioplastics. Microbial production of 3HP from glycerol has previously been …
Adaptive laboratory evolution for growth coupled microbial production
Adaptive laboratory evolution (ALE) is a powerful tool to select for strains with growth-
coupled phenotypes. When coupled with next-generation sequencing and omic …
coupled phenotypes. When coupled with next-generation sequencing and omic …
Metabolic pathway design for growth-associated phenylalanine production using synthetically designed mutualism
Combination of growth-associated pathway engineering based on flux balance analysis
(FBA) and adaptive laboratory evolution (ALE) is a powerful approach to enhance the …
(FBA) and adaptive laboratory evolution (ALE) is a powerful approach to enhance the …
Application of adaptive laboratory evolution to overcome a flux limitation in an Escherichia coli production strain
K Tokuyama, Y Toya, T Horinouchi… - Biotechnology and …, 2018 - Wiley Online Library
Gene deletion strategies using flux balance analysis (FBA) have improved the growth‐
coupled production of various compounds. However, the productivities were often below the …
coupled production of various compounds. However, the productivities were often below the …