Shifting from chemical to biotechnological processes is one of the cornerstones of 21st
century industry. The production of a great range of chemicals via biotechnological means is …

Highlights•Computational tools identify new biological routes to chemicals.•Genome-scale
models suggest genetic engineering strategies to improve production.•Methods that …

Computational modelling of metabolic networks has become an established procedure in
the metabolic engineering of production strains. One key principle that is frequently used to …

Production of chemicals in microorganisms is no longer restricted to products arising from
native metabolic potential. In this review, we highlight the evolution of metabolic engineering …

More than a decade ago, the first genome-scale metabolic models for two of the most
relevant microbes for biotechnology applications, Escherichia coli and Saccaromyces …

Metabolic engineering uses microorganisms to synthesize chemicals from renewable
resources. Given the thousands of known metabolites, it is unclear what valuable chemicals …

Abstract Background 3-hydroxypropionic acid (3HP) is an important chemical precursor for
the production of bioplastics. Microbial production of 3HP from glycerol has previously been …

Adaptive laboratory evolution (ALE) is a powerful tool to select for strains with growth-
coupled phenotypes. When coupled with next-generation sequencing and omic …

Combination of growth-associated pathway engineering based on flux balance analysis
(FBA) and adaptive laboratory evolution (ALE) is a powerful approach to enhance the …

Gene deletion strategies using flux balance analysis (FBA) have improved the growth‐
coupled production of various compounds. However, the productivities were often below the …