Tandem repeats lead to sequence assembly errors and impose multi-level challenges for genome and protein databases

OK Tørresen, B Star, P Mier… - Nucleic acids …, 2019 - academic.oup.com
The widespread occurrence of repetitive stretches of DNA in genomes of organisms across
the tree of life imposes fundamental challenges for sequencing, genome assembly, and …

Widespread deviant patterns of heterozygosity in whole-genome sequencing due to autopolyploidy, repeated elements, and duplication

X Dallaire, R Bouchard, P Hénault… - Genome Biology and …, 2023 - academic.oup.com
Most population genomic tools rely on accurate single nucleotide polymorphism (SNP)
calling and filtering to meet their underlying assumptions. However, genomic complexity …

Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on Ochthebius Beetles from Supratidal Rockpools

AJ García-Meseguer, A Villastrigo, JM Mirón-Gatón… - Insects, 2023 - mdpi.com
Simple Summary Supratidal rockpools stand out as some of the most extreme environments
on the planet. These pools are exposed to the wave's action, fluctuations in sea level …

Genome-wide discovery of single- and multi-locus simple sequence repeat markers and their characterization in Dendrocalamus strictus: a commercial polyploid …

S Rohilla, HS Ginwal, V Rana, S Barthwal… - Genetic Resources and …, 2024 - Springer
Dendrocalamus strictus, popularly known as “lathi baans”, is a multipurpose bamboo
species known for its manifold domestic and industrial applications. In the current scenario …

The salmon louse genome may be much larger than sequencing suggests

GA Wyngaard, R Skern-Mauritzen, K Malde… - Scientific Reports, 2022 - nature.com
The genome size of organisms impacts their evolution and biology and is often assumed to
be characteristic of a species. Here we present the first published estimates of genome size …

Muver, a computational framework for accurately calling accumulated mutations

AB Burkholder, SA Lujan, CA Lavender, SA Grimm… - BMC genomics, 2018 - Springer
Background Identification of mutations from next-generation sequencing data typically
requires a balance between sensitivity and accuracy. This is particularly true of DNA …

DNA-nanopore technology: a human perspective

A Gray - Emerging Topics in Life Sciences, 2021 - portlandpress.com
The purpose of this article is to give a brief overview of the current state of nanopore
sequencing in relation to forensic science with a brief outline of where it stands in relation to …

Development of microsatellite loci and optimization of a multiplex assay for Latibulus argiolus (Hymenoptera: Ichneumonidae), the specialized parasitoid of paper …

A Kostro-Ambroziak, A Siekiera, M Czajkowska… - Scientific Reports, 2020 - nature.com
Microsatellite loci are commonly used markers in population genetic studies. In this study,
we present 40 novel and polymorphic microsatellite loci elaborated for the ichneumonid …

First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity

A Gwiazdowska, O Karpińska… - Scientific Reports, 2021 - nature.com
Abstract The European Robin is a small passerine bird associated with woodlands of
Eurasia and North Africa. Despite being relatively widespread and common, little is known of …

Slippage of the primer strand in the primer extension reaction with modified 2'-deoxyuridine triphosphates

VA Vasiliskov, VE Shershov, RA Miftahov… - Russian Journal of …, 2020 - Springer
An effect of primer strand slippage was observed in the primer extension reaction due to
replacement of thymidine triphosphate for modified analogs of dUTP. The effect was …