Precise spatio–temporal control of gene activity is essential for organismal development,
growth, and survival in a changing environment. Decisive steps in gene regulation involve …

Follicular helper T cells (T FH) are essential B cell-help providers in the formation of
germinal centers (GCs), affinity maturation of GC B cells, differentiation of high-affinity …

Abstract We recently introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an
epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in …

The genome-wide architecture of chromatin-associated proteins that maintains chromosome
integrity and gene regulation is not well defined. Here we use chromatin …

Previously, we described a novel alternative to chromatin immunoprecipitation, CUT&RUN,
in which unfixed permeabilized cells are incubated with antibody, followed by binding of a …

Summary N 6-Methyldeoxyadenosine (6mA) has recently been shown to exist and play
regulatory roles in eukaryotic genomic DNA (gDNA). However, the biological functions of …

The conserved yeast E3 ubiquitin ligase Bre1 and its partner, the E2 ubiquitin-conjugating
enzyme Rad6, monoubiquitinate histone H2B across gene bodies during the transcription …

Gene regulation in eukaryotes requires the controlled access of sequence-specific
transcription factors (TFs) to their sites in a chromatin landscape dominated by …

Regulation of transcription is a tightly choreographed process. The establishment of RNA
polymerase II promoter proximal pausing soon after transcription initiation and the release of …

Origin recognition complex (ORC)-dependent loading of the replicative helicase MCM2-7
onto replication origins in G1-phase forms the basis of replication fork establishment in S …