Systems and synthetic biology-driven engineering of live bacterial therapeutics
The past decade has seen growing interest in bacterial engineering for therapeutically
relevant applications. While early efforts focused on repurposing genetically tractable model …
relevant applications. While early efforts focused on repurposing genetically tractable model …
Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production
L Ricci, A Seifert, S Bernacchi, D Fino… - Microbial …, 2023 - Wiley Online Library
Carbon dioxide (CO2) stands out as sustainable feedstock for developing a circular carbon
economy whose energy supply could be obtained by boosting the production of clean …
economy whose energy supply could be obtained by boosting the production of clean …
Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacterium Eubacterium limosum
Acetogenic bacteria are a unique biocatalyst that highly promises to develop the sustainable
bioconversion of carbon oxides (eg, CO and CO2) into multicarbon biochemicals. Genotype …
bioconversion of carbon oxides (eg, CO and CO2) into multicarbon biochemicals. Genotype …
Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering
PA Sanford, BM Woolston - ACS Synthetic Biology, 2024 - ACS Publications
Eubacterium limosum is a Clostridial acetogen that efficiently utilizes a wide range of single-
carbon substrates and contributes to metabolism of health-associated compounds in the …
carbon substrates and contributes to metabolism of health-associated compounds in the …
Deletion of biofilm synthesis in Eubacterium limosum ATCC 8486 improves handling and transformation efficiency
PA Sanford, KG Miller, KO Hoyt… - FEMS Microbiology …, 2023 - academic.oup.com
Eubacterium limosum is an acetogenic bacterium of potential industrial relevance for its
ability to efficiently metabolize a range of single carbon compounds. However, extracellular …
ability to efficiently metabolize a range of single carbon compounds. However, extracellular …
Expanding the genetic engineering toolbox for the metabolically flexible acetogen Eubacterium limosum
PA Sanford, BM Woolston - Journal of Industrial Microbiology …, 2022 - academic.oup.com
Acetogenic bacteria are an increasingly popular choice for producing fuels and chemicals
from single carbon (C1) substrates. Eubacterium limosum is a promising acetogen with …
from single carbon (C1) substrates. Eubacterium limosum is a promising acetogen with …
Serial adaptive laboratory evolution enhances mixed carbon metabolic capacity of Escherichia coli
Microbes have inherent capacities for utilizing various carbon sources, however they often
exhibit sub-par fitness due to low metabolic efficiency. To test whether a bacterial strain can …
exhibit sub-par fitness due to low metabolic efficiency. To test whether a bacterial strain can …
Strategies in engineering sustainable biochemical synthesis through microbial systems
Y Song, KLJ Prather - Current Opinion in Chemical Biology, 2024 - Elsevier
Growing environmental concerns and the urgency to address climate change have
increased demand for the development of sustainable alternatives to fossil-derived fuels and …
increased demand for the development of sustainable alternatives to fossil-derived fuels and …
[HTML][HTML] Advanced aspects of acetogens
Acetogens are a diverse group of anaerobic bacteria that are capable of carbon dioxide
fixation and have for long fascinated scientists due to their unique metabolic prowess …
fixation and have for long fascinated scientists due to their unique metabolic prowess …
Engineering Acetobacterium wieringae for acetone production from syngas
JPC Moreira, P Montenegro-Silva, JI Alves… - Journal of …, 2024 - Elsevier
Gas fermentation using acetogenic bacteria offers a sustainable alternative to fossil-based
chemical production by converting waste gas streams, such as syngas (CO, H 2, CO 2), into …
chemical production by converting waste gas streams, such as syngas (CO, H 2, CO 2), into …