Systems and synthetic biology-driven engineering of live bacterial therapeutics

K Kim, M Kang, BK Cho - Frontiers in Bioengineering and …, 2023 - frontiersin.org
The past decade has seen growing interest in bacterial engineering for therapeutically
relevant applications. While early efforts focused on repurposing genetically tractable model …

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

L Ricci, A Seifert, S Bernacchi, D Fino… - Microbial …, 2023 - Wiley Online Library
Carbon dioxide (CO2) stands out as sustainable feedstock for developing a circular carbon
economy whose energy supply could be obtained by boosting the production of clean …

Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacterium Eubacterium limosum

J Shin, J Bae, H Lee, S Kang, S Jin… - Proceedings of the …, 2023 - National Acad Sciences
Acetogenic bacteria are a unique biocatalyst that highly promises to develop the sustainable
bioconversion of carbon oxides (eg, CO and CO2) into multicarbon biochemicals. Genotype …

Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering

PA Sanford, BM Woolston - ACS Synthetic Biology, 2024 - ACS Publications
Eubacterium limosum is a Clostridial acetogen that efficiently utilizes a wide range of single-
carbon substrates and contributes to metabolism of health-associated compounds in the …

Deletion of biofilm synthesis in Eubacterium limosum ATCC 8486 improves handling and transformation efficiency

PA Sanford, KG Miller, KO Hoyt… - FEMS Microbiology …, 2023 - academic.oup.com
Eubacterium limosum is an acetogenic bacterium of potential industrial relevance for its
ability to efficiently metabolize a range of single carbon compounds. However, extracellular …

Expanding the genetic engineering toolbox for the metabolically flexible acetogen Eubacterium limosum

PA Sanford, BM Woolston - Journal of Industrial Microbiology …, 2022 - academic.oup.com
Acetogenic bacteria are an increasingly popular choice for producing fuels and chemicals
from single carbon (C1) substrates. Eubacterium limosum is a promising acetogen with …

Serial adaptive laboratory evolution enhances mixed carbon metabolic capacity of Escherichia coli

K Kim, D Choe, M Kang, SH Cho, S Cho, KJ Jeong… - Metabolic …, 2024 - Elsevier
Microbes have inherent capacities for utilizing various carbon sources, however they often
exhibit sub-par fitness due to low metabolic efficiency. To test whether a bacterial strain can …

Strategies in engineering sustainable biochemical synthesis through microbial systems

Y Song, KLJ Prather - Current Opinion in Chemical Biology, 2024 - Elsevier
Growing environmental concerns and the urgency to address climate change have
increased demand for the development of sustainable alternatives to fossil-derived fuels and …

[HTML][HTML] Advanced aspects of acetogens

A Poehlein, B Zeldes, M Flaiz, T Böer, A Lüschen… - Bioresource …, 2024 - Elsevier
Acetogens are a diverse group of anaerobic bacteria that are capable of carbon dioxide
fixation and have for long fascinated scientists due to their unique metabolic prowess …

Engineering Acetobacterium wieringae for acetone production from syngas

JPC Moreira, P Montenegro-Silva, JI Alves… - Journal of …, 2024 - Elsevier
Gas fermentation using acetogenic bacteria offers a sustainable alternative to fossil-based
chemical production by converting waste gas streams, such as syngas (CO, H 2, CO 2), into …