Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

We report on and provide a detailed characterization of the performance and properties of a
recently developed, all Escherichia coli, cell-free transcription and translation system. Gene …

Synthetic biology provides a new paradigm for life science research (“build to learn”) and
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …

CRISPR-Cas systems offer versatile technologies for genome engineering, yet their
implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti …

Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …

The new generation of cell-free gene expression systems enables the prototyping and
engineering of biological systems in vitro over a remarkable scope of applications and …

Synthetic biology concerns the engineering of man‐made living biomachines from
standardized components that can perform predefined functions in a (self‐) controlled …

Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …

Synthetic biology designed cell-free biosensors are a promising new tool for the detection of
clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA …