The process of freezing cells or tissues and depositing them in liquid nitrogen at–196° C is
called cryopreservation. Sub-zero temperature is not a physiological condition for cells and …

This present paper provides an assessment of the occurrence of nitric oxide (NO)-induced
hormetic-biphasic dose/concentration relationships in biomedical research. A substantial …

The whey protein β-lactoglobulin (BLG) is a major milk allergen which is absent in human
milk. Here, we for the first time generated DNA-free BLG bi-allelic knockout cow by zinc …

Human lactoferrin (hLF) is a valuable protein for pharmaceutical products and functional
foods, and worldwide demand for this protein has steadily increased. However, large-scale …

Contents Vitrification has been shown to decrease the developmental capacity of
mammalian oocytes, and this is closely associated with the abnormal mRNA expressions of …

Hydrostatic pressure (HP) and osmotic stress (OS) play an important role in various
biological processes, such as cell proliferation and differentiation. In contrast to canonical …

Induced development of haploid embryos (H) with only paternal (androgenesis) or maternal
(gynogenesis) chromosomes requires irradiation of eggs before fertilization or activation of …

Of all methods exercised in modern molecular biology, modification of cellular properties
through the introduction or removal of nucleic acids is one of the most fundamental. As such …

Research question Can sublethal stress induced by nitric oxide on fresh human
spermatozoa protect the functional properties of post–thaw human spermatozoa? Design …

We evaluated the effect of high hydrostatic pressure on mouse embryonic fibroblasts (MEFs)
and mouse embryonic stem (ES) cells. Hydrostatic pressures of 15, 30, 60, and 90 MPa …