The field of affinity chromatography, which employs a biologically-related agent as the
stationary phase, has seen significant growth since the modern era of this method began in …

The last few decades have witnessed the development of many high-performance
separation methods that use biologically related binding agents. The combination of HPLC …

The physicochemical properties of small molecules that can be determined by retention
measurements in reversed-phase liquid chromatography include solvent-based properties …

α1-Acid glycoprotein (AGP) is a glycoprotein present in serum, which is associated with the
modulation of the immune system in response to stress or injuries, and a biomarker for …

Ligand-target interactions play a central role in drug discovery processes because these
interactions are crucial in biological systems. Small molecules-proteins interactions can …

Ultrafast affinity extraction (UAE) is a form of microscale affinity HPLC that can be employed
to quickly measure equilibrium constants for solute-binding agent interactions in solution …

Quantifying drug-protein interactions has a pivotal role in both early phase drug
development and clinical processes. Diverse affinity chromatographic methods like …

Many drugs bind to serum transport proteins, which can affect both drug distribution and
activity in the body. α 1-Acid glycoprotein (AGP) is a key transport protein for basic and …

A method that combined on-line immunoextraction with high-performance affinity
chromatography was developed to examine the binding of drugs with α 1-acid glycoprotein …

Important information on chemical processes in living systems can be obtained by the rates
at which these biological interactions occur. This review will discuss several techniques …