Roadmap on Label‐Free Super‐Resolution Imaging

VN Astratov, YB Sahel, YC Eldar… - Laser & Photonics …, 2023 - Wiley Online Library
Label‐free super‐resolution (LFSR) imaging relies on light‐scattering processes in
nanoscale objects without a need for fluorescent (FL) staining required in super‐resolved FL …

STED super-resolved microscopy

G Vicidomini, P Bianchini, A Diaspro - Nature methods, 2018 - nature.com
Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while
preserving useful aspects of fluorescence microscopy, such as optical sectioning, and …

Parameter-free image resolution estimation based on decorrelation analysis

A Descloux, KS Grußmayer, A Radenovic - Nature methods, 2019 - nature.com
Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

[HTML][HTML] STED nanoscopy: a glimpse into the future

P Bianchini, C Peres, M Oneto, S Galiani… - Cell and tissue …, 2015 - Springer
The well-known saying of “Seeing is believing” became even more apt in biology when
stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel …

Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED–FCS

E Sezgin, F Schneider, S Galiani, I Urbančič… - Nature protocols, 2019 - nature.com
Super-resolution microscopy techniques enable optical imaging in live cells with
unprecedented spatial resolution. They unfortunately lack the temporal resolution required …

[HTML][HTML] STED nanoscopy with time-gated detection: theoretical and experimental aspects

G Vicidomini, A Schönle, H Ta, KY Han, G Moneron… - PloS one, 2013 - journals.plos.org
In a stimulated emission depletion (STED) microscope the region in which fluorescence
markers can emit spontaneously shrinks with continued STED beam action after a singular …

Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

BR Patton, D Burke, D Owald, TJ Gould… - Optics express, 2016 - opg.optica.org
When imaging through tissue, the optical inhomogeneities of the sample generate
aberrations that can prevent effective Stimulated Emission Depletion (STED) imaging. This …

Circumventing the optical diffraction limit with customized speckles

N Bender, M Sun, H Yılmaz, J Bewersdorf, H Cao - Optica, 2021 - opg.optica.org
Speckle patterns have been used widely in imaging techniques such as ghost imaging,
dynamic speckle illumination microscopy, structured illumination microscopy, and …

[HTML][HTML] From microscopy to nanoscopy via visible light

X Hao, C Kuang, Z Gu, Y Wang, S Li, Y Ku… - Light: Science & …, 2013 - nature.com
The resolution of conventional optical equipment is always restricted by the diffraction limit,
and improving on this was previously considered improbable. Optical super-resolution …

Probing cytoskeletal structures by coupling optical superresolution and AFM techniques for a correlative approach

JV Chacko, FC Zanacchi, A Diaspro - Cytoskeleton, 2013 - Wiley Online Library
In this article, we describe and show the application of some of the most advanced
fluorescence superresolution techniques, STED AFM and STORM AFM microscopy towards …