Change of network structure in agarose gels by aging during storage studied by NMR and electrophoresis

FBA Descallar, S Matsukawa - Carbohydrate polymers, 2020 - Elsevier
Abstract Changes of the network structure in agarose gels during storage were studied by
measuring diffusion coefficient (D) of probe polymers. PGSTE 1H NMR was used to …

Separation of 1–23-kb complementary DNA strands by urea–agarose gel electrophoresis

É Hegedüs, E Kokai, A Kotlyar, V Dombradi… - Nucleic acids …, 2009 - academic.oup.com
Abstract Double-stranded (ds), as well as denatured, single-stranded (ss) DNA samples can
be analyzed on urea–agarose gels. Here we report that after denaturation by heat in the …

A systematic evaluation of the role of crystalline order in nanoporous materials on DNA separation

N Nazemifard, L Wang, W Ye, S Bhattacharjee… - Lab on a Chip, 2012 - pubs.rsc.org
The role of order within a porous separation matrix on the separation efficiency of DNA was
studied systematically. DNA separation was based on a ratchet mechanism. Monodisperse …

Continuum transport model of Ogston sieving in patterned nanofilter arrays for separation of rod‐like biomolecules

ZR Li, GR Liu, YZ Chen, JS Wang, H Bow… - …, 2008 - Wiley Online Library
This article proposes a simple computational transport model of rod‐like short dsDNA
molecules through a microfabricated nanofilter array. Using a nanochannel consisting of …

Transport of biomolecules in asymmetric nanofilter arrays

ZR Li, GR Liu, J Han, YZ Chen, JS Wang… - Analytical and …, 2009 - Springer
We propose a theoretical model for describing the electric-field-driven migration of rod-like
biomolecules in nanofilters comprising a periodic array of shallow passages connecting …

Modeling and experimental characterization of peak tailing in DNA gel electrophoresis

Y Sun, YC Kwok, NT Nguyen - Microfluidics and Nanofluidics, 2007 - Springer
Capillary electrophoresis (CE) is an efficient separation method in analytical chemistry. It
exploits the difference in electrophoretic migration velocities between charged molecular …

Divergent dispersion behavior of ss DNA fragments during microchip electrophoresis in p DMA and LPA entangled polymer networks

CP Fredlake, DG Hert, TP Niedringhaus, JS Lin… - …, 2012 - Wiley Online Library
Resolution of DNA fragments separated by electrophoresis in polymer solutions (“matrices”)
is determined by both the spacing between peaks and the width of the peaks. Prior research …

On-chip fraction collection for multiple selected ssDNA fragments using isolated extraction channels

Z Li, K Sun, M Sunayama, Y Matsuo, V Mizeikis… - … of Chromatography A, 2011 - Elsevier
High efficiency and high-purity fraction collection is highly sought in analysis of fragments-of-
interest from selective polymerase chain reaction (PCR) products generated by High …

Design of separation length and electric field strength for high‐speed DNA electrophoresis

Y Ni, X Dou, S Cheng, Y Zhu - Electrophoresis, 2011 - Wiley Online Library
Gel‐based DNA separation on microchip will play an important role in future genomic
analysis due to its potential for high‐efficiency and high‐speed. Optimal design of microchip …

Electric field gradients during electrophoretic fractionation in microfabricated post arrays

S Kazemlou, N Nazemifard - Microfluidics and nanofluidics, 2014 - Springer
Electrokinetic separation methods have been used extensively in microfluidic devices due to
their separation power and compatibility with miniaturization platform. Electrophoresis (EP) …