Current challenges in designer cellulosome engineering

B Lamote, MJM da Fonseca, J Vanderstraeten… - Applied Microbiology …, 2023 - Springer
Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising
carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high …

Creation of a functional hyperthermostable designer cellulosome

A Kahn, S Moraïs, AP Galanopoulou, D Chung… - Biotechnology for …, 2019 - Springer
Background Renewable energy has become a field of high interest over the past decade,
and production of biofuels from cellulosic substrates has a particularly high potential as an …

Biosynthesis pathways of expanding carbon chains for producing advanced biofuels

H Su, JF Lin - Biotechnology for Biofuels and Bioproducts, 2023 - Springer
Because the thermodynamic property is closer to gasoline, advanced biofuels (C≥ 6) are
appealing for replacing non-renewable fossil fuels using biosynthesis method that has …

Impact of scaffoldin mechanostability on cellulosomal activity

A Galera-Prat, AM Vera, S Moraïs, Y Vazana… - Biomaterials …, 2020 - pubs.rsc.org
Lignocellulose is the most abundant renewable carbon source in the biosphere. However,
the main bottleneck in its conversion to produce second generation biofuels is the …

Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode

J Vanderstraeten, B Lamote, MJM da Fonseca… - Applied Microbiology …, 2022 - Springer
Cellulosomes are multi-enzyme complexes produced by specialised micro-organisms. The
spatial proximity of synergistically acting enzymes incorporated in these naturally occurring …

Cell-surface display of designer cellulosomes by Lactobacillus plantarum

Y Ben-David, S Morais, J Stern, I Mizrahi… - Methods in Enzymology, 2019 - Elsevier
Cell-surface display of designer cellulosomes complexes has attracted increased interest in
recent years. These engineered microorganisms can efficiently degrade lignocellulosic …

Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study

J Vanderstraeten, MJM da Fonseca… - … for biofuels and …, 2022 - Springer
Background Designer cellulosomes are self-assembled chimeric enzyme complexes that
can be used to improve lignocellulosic biomass degradation. They are composed of a …

Cell Surface Engineering: A Fabrication Approach Toward Effective Valorization of Waste

A Binoy, S Singh, S Sadhukhan - Enzymes in the Valorization of …, 2022 - taylorfrancis.com
The generation of the whole-cell biocatalyst is possible by displaying proteins and/or
peptides on the cell surface of yeast or bacteria by genetic engineering. This process is …

Studies on characterization of a novel hemicellulase Abf62A-Axe6A from Ruminiclostridium josui and development of its host-vector system

Y Wang - 2018 - mie-u.repo.nii.ac.jp
From the viewpoint of reduction of the global warming, the utilization and application of the
renewable energies are required. Among various renewable energies, plant biomass is one …