Although fluorescence microscopy is ubiquitous in biomedical research, microscopy
methods reporting is inconsistent and perhaps undervalued. We emphasize the importance …

Fluorescence microscopy has evolved from a purely observational tool to a platform for
quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic …

The optical microscope has revolutionized biology since at least the 17th Century. Since
then, it has progressed from a largely observational tool to a powerful bioanalytical platform …

Recent technological advances have made microscopy indispensable in life science
research. Its ubiquitous use, in turn, underscores the importance of ensuring that microscopy …

Live-cell microscopy is a powerful tool that can reveal cellular behavior as well as the
underlying molecular processes. A key advantage of microscopy is that by visualizing …

In this series of papers on light microscopy imaging, we have covered the fundamentals of
microscopy, super‐resolution microscopy, and lightsheet microscopy. This last review …

The low reproducibility of scientific data published in articles has recently become a cause of
concern in many scientific fields. Data involving light microscopy is no exception. The low …

Fluorescence microscopy images should not be treated as perfect representations of
biology. Many factors within the biospecimen itself can drastically affect quantitative …

The visual allure of microscopy makes it an intuitively powerful research tool. Intuition,
however, can easily obscure or distort the reality of the information contained in an image …

Modern life science research is a collaborative effort. Few research groups can single‐
handedly support the necessary equipment, expertise and personnel needed for the ever …