Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

Lignin conversion to β-ketoadipic acid by Pseudomonas putida via metabolic engineering and bioprocess development

AZ Werner, WT Cordell, CW Lahive, BC Klein… - Science …, 2023 - science.org
Bioconversion of a heterogeneous mixture of lignin-related aromatic compounds (LRCs) to a
single product via microbial biocatalysts is a promising approach to valorize lignin. Here …

[HTML][HTML] A field of dreams: Lignin valorization into chemicals, materials, fuels, and health-care products

J Becker, C Wittmann - Biotechnology Advances, 2019 - Elsevier
Lignin is one of the most abundant renewable resources on earth and is readily produced as
a sidestream during biomass fractioning. So far, these large quantities of lignin have been …

High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration

JR Elmore, GN Dexter, H Baldino, JD Huenemann… - Science …, 2023 - science.org
Efficient genome engineering is critical to understand and use microbial functions. Despite
recent development of tools such as CRISPR-Cas gene editing, efficient integration of …

Chem-bio interface design for rapid conversion of CO2 to bioplastics in an integrated system

P Zhang, K Chen, B Xu, J Li, C Hu, JS Yuan, SY Dai - Chem, 2022 - cell.com
Integrating catalytic CO 2 reduction with bioconversion could substantially advance carbon
capture and utilization and mitigate climate change. However, the state-of-the-arts are …

Recent progress in the synthesis of advanced biofuel and bioproducts

BF Pfleger, R Takors - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights●Why biofuels and bioproducts remain critical to society●What molecular
products can be made by biotechnology using available feedstocks●How new systems and …

Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis

S Thorwall, C Schwartz, JW Chartron… - Nature chemical …, 2020 - nature.com
Microbial chemical production is a rapidly growing industry, with much of the growth fueled
by advances in synthetic biology. New approaches have enabled rapid strain engineering …

Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440

GJ Bentley, N Narayanan, RK Jha, D Salvachúa… - Metabolic …, 2020 - Elsevier
Pseudomonas putida KT2440 has received increasing attention as an important biocatalyst
for the conversion of diverse carbon sources to multiple products, including the olefinic …

Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection

NT Wirth, E Kozaeva, PI Nikel - Microbial Biotechnology, 2020 - Wiley Online Library
Pseudomonas species have become reliable platforms for bioproduction due to their
capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their …