The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

For centuries, cell biology has been based on light microscopy and at the same time been
limited by its optical resolution. However, several new technologies have been developed …

Anyone who has used a light microscope has wished that its resolution could be a little
better. Now, after centuries of gradual improvements, fluorescence microscopy has made a …

Abstract In 1873, Ernst Abbe discovered that features closer than∼ 200 nm cannot be
resolved by lens-based light microscopy. In recent years, however, several new far-field …

Until not very long ago, it was widely accepted that lens-based (far-field) optical microscopes
cannot visualize details much finer than about half the wavelength of light. The advent of …

We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick
specimens (50–150 μm), by coupling far-field individual molecule localization with selective …

Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

This paper presents a review of imaging techniques and of their utility in system biology.
During the last decade systems biology has matured into a distinct field and imaging has …

Since the work of Golgi and Cajal, light microscopy has remained a key tool for
neuroscientists to observe cellular properties. Ongoing advances have enabled new …

Fluorescent markers emitting in the red are extremely valuable in biological microscopy
since they minimize cellular autofluorescence and increase flexibility in multicolor …