Biotin-dependent carboxylases include acetyl-CoA carboxylase (ACC), propionyl-CoA
carboxylase (PCC), 3-methylcrotonyl-CoA carboxylase (MCC), geranyl-CoA carboxylase …

The activity of the biotin-dependent enzyme pyruvate carboxylase from many organisms is
highly regulated by the allosteric activator acetyl-CoA. A number of X-ray crystallographic …

Pyruvate carboxylase (PC) catalyzes the two-step carboxylation of pyruvate to produce
oxaloacetate, playing a key role in the maintenance of metabolic homeostasis in cells. Given …

The objective of our study was to determine the mechanism of action of the short-chain
ceramide analog, C6-ceramide, and the breast cancer drug, tamoxifen, which we show …

Pyruvate carboxylase (PC) catalyzes the ATP-dependent carboxylation of pyruvate to
oxaloacetate, an important anaplerotic reaction in mammalian tissues. To effect catalysis …

Pyruvate carboxylase is a metabolic enzyme that fuels the tricarboxylic acid cycle with one of
its intermediates and also participates in the first step of gluconeogenesis. This large …

The third-generation (3G) biorefinery aims to use microbial cell factories or enzymatic
systems to synthesize value-added chemicals from one-carbon (C1) sources, such as CO2 …

Pyruvate carboxylase (PC) catalyzes the ATP-dependent carboxylation of pyruvate to
oxaloacetate. The reaction occurs in two separate catalytic domains, coupled by the long …

Biotin-dependent enzymes catalyze carboxyl transfer reactions by efficiently coordinating
multiple reactions between spatially distinct active sites. Pyruvate carboxylase (PC), a …

The catalytic mechanism of the MgATP-dependent carboxylation of biotin in the biotin
carboxylase domain of pyruvate carboxylase from R. etli (Re PC) is common to the biotin …