Asymmetric lipid membranes: towards more realistic model systems

D Marquardt, B Geier, G Pabst - Membranes, 2015 - mdpi.com
Despite the ubiquity of transbilayer asymmetry in natural cell membranes, the vast majority
of existing research has utilized chemically well-defined symmetric liposomes, where the …

Interleaflet coupling, pinning, and leaflet asymmetry—major players in plasma membrane nanodomain formation

T Fujimoto, I Parmryd - Frontiers in cell and developmental biology, 2017 - frontiersin.org
The plasma membrane has a highly asymmetric distribution of lipids and contains dynamic
nanodomains many of which are liquid entities surrounded by a second, slightly different …

Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins

R Raghupathy, AA Anilkumar, A Polley, PP Singh… - Cell, 2015 - cell.com
Understanding how functional lipid domains in live cell membranes are generated has
posed a challenge. Here, we show that transbilayer interactions are necessary for the …

GPI-anchored protein organization and dynamics at the cell surface

S Saha, AA Anilkumar, S Mayor - Journal of lipid research, 2016 - ASBMB
The surface of eukaryotic cells is a multi-component fluid bilayer in which
glycosylphosphatidylinositol (GPI)-anchored proteins are an abundant constituent. In this …

Intrinsic curvature-mediated transbilayer coupling in asymmetric lipid vesicles

B Eicher, D Marquardt, FA Heberle, I Letofsky-Papst… - Biophysical journal, 2018 - cell.com
We measured the effect of intrinsic lipid curvature, J 0, on structural properties of asymmetric
vesicles made of palmitoyl-oleoyl-phosphatidylethanolamine (POPE; J 0< 0) and palmitoyl …

Model membrane systems used to study plasma membrane lipid asymmetry

HL Scott, KB Kennison, TA Enoki, M Doktorova… - Symmetry, 2021 - mdpi.com
It is well known that the lipid distribution in the bilayer leaflets of mammalian plasma
membranes (PMs) is not symmetric. Despite this, model membrane studies have largely …

Transbilayer colocalization of lipid domains explained via measurement of strong coupling parameters

MC Blosser, AR Honerkamp-Smith, T Han, M Haataja… - Biophysical journal, 2015 - cell.com
When micron-scale compositional heterogeneity develops in membranes, the distribution of
lipids on one face of the membrane strongly affects the distribution on the other. Specifically …

Lipid Acyl Chain cis Double Bond Position Modulates Membrane Domain Registration/Anti-Registration

S Zhang, X Lin - Journal of the American Chemical Society, 2019 - ACS Publications
Inter-leaflet coupling is critical to control the dynamics of membrane domain registration/anti-
registration, which is important in maintaining proper biological functions. Factors such as …

Roles of interleaflet coupling and hydrophobic mismatch in lipid membrane phase-separation kinetics

PW Fowler, JJ Williamson, MSP Sansom… - Journal of the …, 2016 - ACS Publications
Characterizing the nanoscale dynamic organization within lipid bilayer membranes is
central to our understanding of cell membranes at a molecular level. We investigate phase …

Live cell plasma membranes do not exhibit a miscibility phase transition over a wide range of temperatures

IH Lee, S Saha, A Polley, H Huang… - The Journal of …, 2015 - ACS Publications
Lipid/cholesterol mixtures derived from cell membranes as well as their synthetic
reconstitutions exhibit well-defined miscibility phase transitions and critical phenomena near …