Digital PCR: modern solution to parasite diagnostics and population trait genetics

P Baltrušis, J Höglund - Parasites & Vectors, 2023 - Springer
The use of polymerase chain reaction (PCR)-based diagnostic approaches has steadily
increased in the field of parasitology in recent decades. The most recent large-scale …

Bangladesh in the era of malaria elimination

K Haldar, MS Alam, C Koepfli, NF Lobo, CS Phru… - Trends in …, 2023 - cell.com
Bangladesh has dramatically reduced malaria by 93% from 2008 to 2020. The strategy has
been district-wise, phased elimination; however, the last districts targeted for elimination …

A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food

Y Zhao, W Wu, X Tang, Q Zhang, J Mao, L Yu… - Biosensors and …, 2023 - Elsevier
Growing studies focusing on nuclear acid detection via the emerging CRISPR technique
demonstrate its promising application. However, limited works solve the identification of non …

Accuracy of diagnosis among clinical malaria patients: comparing microscopy, RDT and a highly sensitive quantitative PCR looking at the implications for …

S Opoku Afriyie, TK Addison, Y Gebre, AH Mutala… - Malaria Journal, 2023 - Springer
Abstract Background The World Health Organization recommends parasitological
confirmation of all suspected malaria cases by microscopy or rapid diagnostic tests (RDTs) …

A Countrywide Survey of hrp2/3 Deletions and kelch13 Mutations Co-occurrence in Ethiopia

C Kamaliddin, J Burke-Gaffney, S Ashraf… - The Journal of …, 2024 - academic.oup.com
Malaria elimination relies on detection of Plasmodium falciparum histidine-rich proteins 2/3
(HRP2/3) through rapid diagnostic tests (RDTs) and treatment with artemisinin combination …

Flexible and cost-effective genomic surveillance of P. falciparum malaria with targeted nanopore sequencing

M de Cesare, M Mwenda, AE Jeffreys, J Chirwa… - Nature …, 2024 - nature.com
Genomic surveillance of Plasmodium falciparum malaria can provide policy-relevant
information about antimalarial drug resistance, diagnostic test failure, and the evolution of …

Risk of Malaria Following Untreated Subpatent Plasmodium falciparum Infections: Results Over 4 Years From a Cohort in a High-Transmission Area in Western …

EE Zeno, AA Obala, B Pence… - The Journal of …, 2024 - academic.oup.com
Background People with suspected malaria may harbor Plasmodium falciparum undetected
by rapid diagnostic test (RDT). The impact of these subpatent infections on the risk of …

Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana

TA Kayode, AK Addo, TK Addison, A Tweneboah… - Malaria Journal, 2024 - Springer
Background Accurate diagnosis and timely treatment are crucial in combating malaria.
Methods A total of 449 samples were screened for Plasmodium falciparum infection by …

Evaluation of an ultrasensitive HRP2–based rapid diagnostic test for detection of asymptomatic Plasmodium falciparum parasitaemia among children in western …

LB Turnbull, G Ayodo, V Knight, CC John, MS McHenry… - Malaria Journal, 2022 - Springer
Background Accurate detection of asymptomatic malaria parasitaemia in children living in
high transmission areas is important for malaria control and reduction programmes that …

Low Prevalence of Plasmodium falciparum Histidine-Rich Protein 2 and 3 Gene Deletions—A Multiregional Study in Central and West Africa

T Krueger, M Ikegbunam, A Lissom, TL Sandri… - Pathogens, 2023 - mdpi.com
Plasmodium falciparum parasites carrying deletions of histidine-rich protein 2 and 3 genes,
pfhrp2 and pfhrp3, respectively, are likely to escape detection via HRP2-based rapid …