The carboxy-terminal domain (CTD) extends from the largest subunit of RNA polymerase II
(Pol II) as a long, repetitive and largely unstructured polypeptide chain. Throughout the …

Several macromolecular machines collaborate to produce eukaryotic messenger RNA. RNA
polymerase II (Pol II) translocates along genes that are up to millions of base pairs in length …

Histone acetylation is a ubiquitous hallmark of transcription, but whether the link between
histone acetylation and transcription is causal or consequential has not been addressed …

The C-terminal domain (CTD) of the large subunit of RNA polymerase (pol) II comprises
conserved heptad repeats, and post-translational modification of the CTD regulates …

Gene regulation is a dynamic process involving changes ranging from the remodeling of
chromatin to preferential translation. To understand integrated nuclear and cytoplasmic …

At each active protein-encoding gene, nascent RNA is tethered to the DNA axis by
elongating RNA polymerase II (Pol II) and is continuously altered by splicing and other …

Transcription elongation rates influence RNA processing, but sequence-specific regulation
is poorly understood. We addressed this in vivo, analyzing RNAPI in S. cerevisiae. Mapping …

Termination of RNA polymerase II (Pol II) transcription is a key step that is important for 3′
end formation of functional mRNA, mRNA release, and Pol II recycling. Even so, the …

The dynamics of transcription can be studied genome wide by high-throughput sequencing
of nascent and newly synthesized RNA. 4-thiouridine (4SU) labeling in vivo enables the …

Eukaryotic cells synthesize enormous quantities of RNA from diverse classes, most of which
are subject to extensive processing. These processes are inherently error-prone, and cells …