The primary mechanisms by which bacteria lose viability when deprived of thymine have
been elusive for over half a century. Early research focused on stalled replication forks and …

The origins of Molecular Biology and Bacterial Physiology are reviewed, from our personal
standpoints, emphasizing the coupling between bacterial growth, chromosome replication …

Background Toxic compounds present in both the hydrolysate and pyrolysate of
lignocellulosic biomass severely hinder the further conversion of lignocellulose-derived …

Starvation of cells for the DNA building block dTTP is strikingly lethal (thymineless death,
TLD), and this effect is observed in all organisms. The phenomenon, discovered some 60 …

The Escherichia coli dgt gene encodes a dGTP triphosphohydrolase whose detailed role
still remains to be determined. Deletion of dgt creates a mutator phenotype, indicating that …

Our laboratory recently discovered that Escherichia coli cells starved for the DNA precursor
dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for …

Thymineless death (TLD) is the rapid loss of colony-forming ability in bacterial, yeast and
human cells starved for thymine, and is the mechanism of action of common …

ABSTRACT dGTP starvation, a newly discovered phenomenon in which Escherichia coli
cells are starved specifically for the DNA precursor dGTP, leads to impaired growth and …

The three main chapters of this thesis (Ch2, Ch3, Ch4) address the topics of antibiotic
resistance, persistence and thymineless death respectively. The antibiotic resistance …

Background: Spotted Fever group rickettsiae are obligate intracellular arthropod-borne
bacteria. Rickettsiae are globally distributed yet typically confined to the distribution of their …