Applying CRISPR/Cas for genome engineering in plants: the best is yet to come

H Puchta - Current opinion in plant biology, 2017 - Elsevier
Highlights•CRISPR/Cas mediated gene editing is becoming routine for plants.•Much more
can be achieved in future by further applications.•Gene editing will become more precise …

The contributions of transposable elements to the structure, function, and evolution of plant genomes

JL Bennetzen, H Wang - Annual review of plant biology, 2014 - annualreviews.org
Transposable elements (TEs) are the key players in generating genomic novelty by a
combination of the chromosome rearrangements they cause and the genes that come under …

The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable …

S Schiml, F Fauser, H Puchta - The Plant Journal, 2014 - Wiley Online Library
The CRISPR/C as nuclease is becoming a major tool for targeted mutagenesis in
eukaryotes by inducing double‐strand breaks (DSB s) at pre‐selected genomic sites that are …

DNA damage and repair in plants–from models to crops

V Manova, D Gruszka - Frontiers in plant science, 2015 - frontiersin.org
The genomic integrity of every organism is constantly challenged by endogenous and
exogenous DNA-damaging factors. Mutagenic agents cause reduced stability of plant …

Molecular Poltergeists: Mitochondrial DNA Copies (numts) in Sequenced Nuclear Genomes

E Hazkani-Covo, RM Zeller, W Martin - PLoS genetics, 2010 - journals.plos.org
The natural transfer of DNA from mitochondria to the nucleus generates nuclear copies of
mitochondrial DNA (numts) and is an ongoing evolutionary process, as genome sequences …

The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution

H Puchta - Journal of experimental botany, 2005 - academic.oup.com
The efficient repair of double-strand breaks (DSBs) in genomic DNA is important for the
survival of all organisms. In recent years, basic mechanisms of DSB repair in somatic plant …

Architecture and evolution of a minute plant genome

E Ibarra-Laclette, E Lyons, G Hernández-Guzmán… - Nature, 2013 - nature.com
It has been argued that the evolution of plant genome size is principally unidirectional and
increasing owing to the varied action of whole-genome duplications (WGDs) and mobile …

Advances in the study of polyploidy since Plant speciation

DE Soltis, PS Soltis, JA Tate - New phytologist, 2004 - Wiley Online Library
Enormous strides have been made in the study of polyploidy over the last 20 yr. Here, we
highlight some of these discoveries and note where our understanding of polyploid …

CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination

TK Huang, H Puchta - Plant Cell Reports, 2019 - Springer
Key message We summarize recent progress of CRISPR/Cas9-mediated gene targeting in
plants, provide recommendations for designing gene-targeting vectors and highlight the …

Genome size reduction through illegitimate recombination counteracts genome expansion in Arabidopsis

KM Devos, JKM Brown, JL Bennetzen - Genome research, 2002 - genome.cshlp.org
Genome size varies greatly across angiosperms. It is well documented that, in addition to
polyploidization, retrotransposon amplification has been a major cause of genome …