Dengue virus (DENV) is a member of the Flavivirus genus of the Flaviviridae family of
enveloped, positive-strand RNA viruses. The Flavivirus genus includes viruses transmitted …

Among animal viruses, arboviruses are unique in that they depend on arthropod vectors for
transmission. Field research and laboratory investigations related to the three components …

How viruses evolve within hosts can dictate infection outcomes; however, reconstructing this
process is challenging. We evaluate our multiplexed amplicon approach, PrimalSeq, to …

Dengue is a major international public health concern, and the number of outbreaks has
escalated greatly. Human migration and international trade and travel are constantly …

Background Extensive genetic diversity in viral populations within infected hosts and the
divergence of variants from existing reference genomes impede the analysis of deep viral …

Background Several observations support the hypothesis that vector-driven selection plays
an important role in shaping dengue virus (DENV) genetic diversity. Clustering of DENV …

Due to their error-prone replication, RNA viruses typically exist as a diverse population of
closely related genomes, which is considered critical for their fitness and adaptive potential …

Intrahost genetic diversity was analysed in naturally infected mosquitoes and birds to
determine whether West Nile virus (WNV) exists in nature as a quasispecies and to quantify …

The evolutionary dynamics of a virus can differ within hosts and across populations. Studies
of within-host evolution provide an important link between experimental studies of virus …

Background Massively parallel sequencing offers the possibility of revolutionizing the study
of viral populations by providing ultra deep sequencing (tens to hundreds of thousand fold …