Thanks to the synthetic biology, the laborious and restrictive procedure for producing a
target protein in living microorganisms by biotechnological approaches can now experience …

Microfluidic, as the systems for using microchannel (micron-or sub-micron scale) to process
or manipulate microflow, is being widely applied in enzyme biotechnology and biocatalysis …

PEGylation is a well-established and clinically proven half-life extension strategy for protein
delivery. Protein modification with amine-reactive poly (ethylene glycol)(PEG) generates …

The reliance of pharmaceutical production on batch reactors dating back to the 20th century
has exposed vulnerabilities in the supply chains of essential medicines, which were …

PEGylation of therapeutic proteins has long been recognized as a safe and effective
approach to enhance pharmacokinetic properties of proteins by increasing the in-vivo half …

In this study, a continuous flow microchannel reactor was designed and constructed for
continuous and efficient fatty acid chain modification (FACylation) of polypeptides to achieve …

Protein PEGylation is a traditional bioconjugation technology that enhances the therapeutic
efficacy and in vivo half-life of proteins by the formation of covalent bonds with highly …

Protein engineering has gained significant attention worldwide. Cell-based protein synthesis
is tedious and takes a long time to synthesize the desired protein. Microfluidics is an …

The present invention provides a novel process of PEGy lation and purification of the
PEGylated therapeutic pro teins. The developed PEGylation protocol provides high yield of …