The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

Cancer is driven by multiple types of genetic alterations, which range in size from point
mutations to whole-chromosome gains and losses, known as aneuploidy. Chromosome …

We investigated genome folding across the eukaryotic tree of life. We find two types of three-
dimensional (3D) genome architectures at the chromosome scale. Each type appears and …

The Hi-C technique has been shown to be a promising method to detect structural variations
(SVs) in human genomes. However, algorithms that can use Hi-C data for a full-range SV …

Variants of uncertain significance fundamentally limit the clinical utility of genetic information.
The challenge they pose is epitomized by BRCA1, a tumour suppressor gene in which …

We present single-cell combinatorial indexed Hi-C (sciHi-C), a method that applies
combinatorial cellular indexing to chromosome conformation capture. In this proof of …

Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a
powerful genome-editing method that facilitates genetic alterations of genomes in a variety …

The prokaryotic type II CRISPR–Cas9 (clustered regularly interspaced short palindromic
repeats–CRISPR-associated 9) system is rapidly revolutionizing the field of genetic …

The epidermal growth factor receptor (EGFR) is frequently mutated in human cancer,, and is
an important therapeutic target. EGFR inhibitors have been successful in lung cancer, where …

Genome-editing tools, and in particular those based on CRISPR–Cas (clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated protein) systems, are …