The first disulfide linkage-employing drug conjugate that exploits the reversible nature of this
unique covalent bond was recently approved for human use. Increasing numbers of drug …

Eukaryotic life depends on the spatial and temporal organization of cellular membrane
systems. Recent advances in understanding the machinery of vesicle transport have …

Transport of membranes and proteins in eukaryotic cells is mediated by vesicular carriers.
Here we review the biogenesis and functions of COPI vesicles, carriers that operate in the …

Pseudomonas exotoxin A (PE) is a highly toxic protein secreted by the opportunistic
pathogen Pseudomonas aeruginosa. The modular structure and corresponding mechanism …

Exocytic transport from the endoplasmic reticulum (ER) to the Golgi complex has been
visualized in living cells using a chimera of the temperature-sensitive glycoprotein of …

Coronaviruses (CoVs) assemble by budding into the lumen of the intermediate compartment
(IC) at the endoplasmic reticulum (ER)-Golgi interface. However, why CoVs have chosen the …

Poliovirus RNA replicative complexes are associated with cytoplasmic membranous
structures that accumulate during viral infection. These membranes were immunoisolated by …

Electron microscope immunocytochemistry reveals that both anterograde-directed
(proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are …

Selective export and retrieval of proteins between the endoplasmic reticulum (ER) and Golgi
apparatus is indispensable for eukaryotic cell function. An essential step in the retrieval of …

Each organelle of the secretory pathway is required to selectively allow transit of newly
synthesized secretory and plasma membrane proteins and also to maintain a unique set of …