The synthetic biology toolbox for tuning gene expression in yeast

H Redden, N Morse, HS Alper - FEMS yeast research, 2015 - search.proquest.com
Saccharomyces cerevisiae can serve as a key production platform for biofuels,
nutraceuticals, industrial compounds, and therapeutic proteins. Over the recent years …

Glycerol export and glycerol-3-phosphate dehydrogenase, but not glycerol phosphatase, are rate limiting for glycerol production in Saccharomyces cerevisiae

F Remize, L Barnavon, S Dequin - Metabolic engineering, 2001 - Elsevier
Glycerol, one of the most important by-products of alcoholic fermentation, has positive effects
on the sensory properties of fermented beverages. It was recently shown that the most direct …

Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex

SS Patel, BJ Belmont, JM Sante, MF Rexach - Cell, 2007 - cell.com
Nuclear pore complexes (NPCs) form aqueous conduits in the nuclear envelope and gate
the diffusion of large proteins between the cytoplasm and nucleoplasm. NPC proteins …

[HTML][HTML] A disulfide relay system in the intermembrane space of mitochondria that mediates protein import

N Mesecke, N Terziyska, C Kozany, F Baumann… - Cell, 2005 - cell.com
We describe here a pathway for the import of proteins into the intermembrane space (IMS) of
mitochondria. Substrates of this pathway are proteins with conserved cysteine motifs, which …

Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast.

JW Riesmeier, L Willmitzer, WB Frommer - The EMBO Journal, 1992 - embopress.org
Active loading of the phloem with sucrose in leaves is an essential part of the process of
supplying non‐photosynthetic tissues with carbon and energy. The transport is protein …

Mitochondria‐targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae

B Westermann, W Neupert - Yeast, 2000 - Wiley Online Library
We describe the construction and characterization of a novel set of plasmids for expression
of mitochondria‐targeted green fluorescent protein (GFP) in Saccharomyces cerevisiae. The …

Yeast Vps13 promotes mitochondrial function and is localized at membrane contact sites

JS Park, MK Thorsness, R Policastro… - Molecular biology of …, 2016 - Am Soc Cell Biol
The Vps13 protein family is highly conserved in eukaryotic cells. Mutations in human VPS13
genes result in a variety of diseases, such as chorea acanthocytosis (ChAc), but the cellular …

Three Arabidopsis fatty acyl-coenzyme A reductases, FAR1, FAR4, and FAR5, generate primary fatty alcohols associated with suberin deposition

F Domergue, SJ Vishwanath, J Joubès, J Ono… - Plant …, 2010 - academic.oup.com
Suberin is a protective hydrophobic barrier consisting of phenolics, glycerol, and a variety of
fatty acid derivatives, including C18: 0-C22: 0 primary fatty alcohols. An eight-member gene …

[HTML][HTML] Mutations that suppress the thermosensitivity of green fluorescent protein

KR Siemering, R Golbik, R Sever, J Haseloff - Current Biology, 1996 - cell.com
Background The green fluorescent protein (GFP) of the jellyfish Aequorea victoria has
recently attracted great interest as the first example of a cloned reporter protein that is …

Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth

J Zhang, H Liu, J Sun, B Li, Q Zhu, S Chen, H Zhang - PloS one, 2012 - journals.plos.org
Fatty acid desaturases play important role in plant responses to abiotic stresses. However,
their exact function in plant resistance to salt stress is unknown. In this work, we provide the …