Dynamic Nuclear Polarization Illuminates Key Protein–Lipid Interactions in the Native Bacterial Cell Envelope

JE Kent, BE Ackermann, GT Debelouchina… - Biochemistry, 2023 - ACS Publications
Elucidating the structure and interactions of proteins in native environments is a fundamental
goal of structural biology. Nuclear magnetic resonance (NMR) spectroscopy is well suited for …

A Comparative Study of Nitroxide‐Based Biradicals for Dynamic Nuclear Polarization in Cellular Environments

BE Ackermann, BJ Lim, N Elathram… - …, 2022 - Wiley Online Library
Dynamic nuclear polarization (DNP) is a powerful tool to enhance the NMR signals of
molecules by transferring polarization from unpaired electron spins to nuclei through …

Solid-state NMR MAS CryoProbe enables structural studies of human blood protein vitronectin bound to hydroxyapatite

T Gopinath, K Shin, Y Tian, W Im, J Struppe… - Journal of Structural …, 2024 - Elsevier
The low sensitivity of nuclear magnetic resonance (NMR) is a major bottleneck for studying
biomolecular structures of complex biomolecular assemblies. Cryogenically cooled probe …

Interaction between Yersinia pestis Ail Outer Membrane Protein and the C-Terminal Domain of Human Vitronectin

L Vasseur, F Barbault, A Monari - The Journal of Physical …, 2024 - ACS Publications
Yersinia pestis, the causative agent of plague, is capable of evading the human immune
system response by recruiting the plasma circulating vitronectin proteins, which act as a …

Interplays of mutations in waaA, cmk, and ail contribute to phage resistance in Yersinia pestis

L Xiao, Z Qi, K Song, R Lv, R Chen, H Zhao… - Frontiers in Cellular …, 2023 - frontiersin.org
Plague caused by Yersinia pestis remains a public health threat worldwide. Because
multidrug-resistant Y. pestis strains have been found in both humans and animals, phage …

Membranes, Minerals and Magnets: Application of NMR Spectroscopy to Biological Interfaces

JE Kent, K Shin, FM Marassi - 2022 - books.rsc.org
Nature has evolved sophisticated mechanisms of producing biomolecular structures that
compartmentalize various biochemical and cellular environments. Biological membranes …

Structure-and Dynamics-guided Drug Development Using NMR and its Application to Diverse Pharmaceutical Modalities

K Takeuchi - 2022 - books.rsc.org
The unique strength of solution-state nuclear magnetic resonance (NMR) is to contribute not
only to determine high-resolution structures of macromolecules but also to provide …